Optogenetic control of Nodal signaling patterns

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dc.catalogadorgjm
dc.contributor.authorMcNamara, Harold M.
dc.contributor.authorJia, Bill Z.
dc.contributor.authorGuyer, Alison
dc.contributor.authorParot Fernández, Vicente José
dc.contributor.authorDobbs, Caleb
dc.contributor.authorSchier, Alexander F.
dc.contributor.authorCohen, Adam E.
dc.contributor.authorLord, Nathan D.
dc.date.accessioned2024-04-17T14:27:25Z
dc.date.available2024-04-17T14:27:25Z
dc.date.issued2024
dc.description.abstractA crucial step in early embryogenesis is the establishment of spatial patterns of signaling activity. Tools to perturb morphogen signals with high resolution in space and time can help reveal how embryonic cells decode these signals to make appropriate fate decisions. Here, we present new optogenetic reagents and an experimental pipeline for creating designer Nodal signaling patterns in live zebrafish embryos. Nodal receptors were fused to the light-sensitive heterodimerizing pair Cry2/CIB1N, and the Type II receptor was sequestered to the cytosol. The improved optoNodal2 reagents eliminate dark activity and improve response kinetics, without sacrificing dynamic range. We adapted an ultra-widefield microscopy platform for parallel light patterning in up to 36 embryos and demonstrated precise spatial control over Nodal signaling activity and downstream gene expression. Patterned Nodal activation drove precisely controlled internalization of endodermal precursors. Further, we used patterned illumination to generate synthetic signaling patterns in Nodal signaling mutants, rescuing several characteristic developmental defects. This study establishes an experimental toolkit for systematic exploration of Nodal signaling patterns in live embryos.
dc.fechaingreso.objetodigital2024-04-17
dc.format.extent26 páginas
dc.fuente.origenORCID
dc.identifier.doi10.1101/2024.04.11.588875
dc.identifier.urihttps://doi.org/10.1101/2024.04.11.588875
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/85155
dc.information.autorucInstituto de Ingeniería Biológica y Médica; Parot Fernández, Vicente José; 0000-0002-9261-6667; 140998
dc.language.isoen
dc.nota.accesocontenido completo
dc.rightsacceso abierto
dc.rights.licenseCC BY-NC-ND 4.0 DEED Attribution-NonCommercial-NoDerivs 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleOptogenetic control of Nodal signaling patterns
dc.typepreprint
sipa.codpersvinculados140998
sipa.trazabilidadORCID;2024-04-15
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