Efficient <i>in vitro</i> multiplication of <i>Aechmea</i> 'Little Harv' and <i>Tillandsia cyanea</i> Linden ex K. Koch
| dc.contributor.author | Cueva, Augusta | |
| dc.contributor.author | Espinosa, Carlos | |
| dc.contributor.author | Jordan, Miguel | |
| dc.date.accessioned | 2025-01-21T01:05:47Z | |
| dc.date.available | 2025-01-21T01:05:47Z | |
| dc.date.issued | 2006 | |
| dc.description.abstract | In vitro plant regeneration of Aechmea 'Little Harv' and Tillandsia cyanea, (Bromeliaceae), two endangered and important ornamental bromeliads native to Ecuador was achieved from seedling explants. T cyanea seedlings germinated or underwent indirect shoot organogenesis from callus when cultivated in a Murashige and Skoog (1962) nutrient medium in the presence of various levels of NAA (0.5 or 1.0 mg 1(-1)) in combination with BAP (0.1, 0.5 or 1.0 mg 1(-1)), plus 40 mg 1(-1) adenine. A. 'Little Harv' seed explants formed adventitious shoots without callus initiation. In both plants, the best shoot regeneration (approx. 9-10 shoots/explant) was obtained using 10 mg 1(-1) NAA and 0.1 mg 1(-1) BAP, in combination with adenine, but other growth regulator combinations were also successful. In the case of Aechmea, the addition of IAA instead of NAA was less efficient (2 shoots/explant). In addition to shoot formation 1 mg 1(-1) NAA and 0.1 mg 1(-1) induced callus on approx. 30% of T cyanea and 8% of A. 'Little Harv' explants. Isolated shoots formed roots after subculture in growth regulator-free media. | |
| dc.fuente.origen | WOS | |
| dc.identifier.issn | 1311-9109 | |
| dc.identifier.uri | https://repositorio.uc.cl/handle/11534/96016 | |
| dc.identifier.wosid | WOS:000242955100002 | |
| dc.issue.numero | 4 | |
| dc.language.iso | en | |
| dc.pagina.final | 169 | |
| dc.pagina.inicio | 165 | |
| dc.revista | Propagation of ornamental plants | |
| dc.rights | acceso restringido | |
| dc.subject | adenine | |
| dc.subject | endangered bromeliads | |
| dc.subject | in vitro regeneration | |
| dc.subject | ornamental bromeliads | |
| dc.subject | shoot organogenesis | |
| dc.subject.ods | 15 Life on Land | |
| dc.subject.odspa | 15 Vida de ecosistemas terrestres | |
| dc.title | Efficient <i>in vitro</i> multiplication of <i>Aechmea</i> 'Little Harv' and <i>Tillandsia cyanea</i> Linden ex K. Koch | |
| dc.type | artículo | |
| dc.volumen | 6 | |
| sipa.index | WOS | |
| sipa.trazabilidad | WOS;2025-01-12 |