Oral vitamin D modulates the epidermal expression of the vitamin D receptor and cathelicidin in children with atopic dermatitis

dc.catalogadorjca
dc.contributor.authorCabalín, Carolina
dc.contributor.authorPérez-Mateluna, Guillermo
dc.contributor.authorIturriaga, Carolina
dc.contributor.authorCamargo Jr., Carlos A.
dc.contributor.authorBorzutzky Schachter, Arturo
dc.date.accessioned2023-01-19T14:15:18Z
dc.date.available2023-01-19T14:15:18Z
dc.date.issued2022
dc.description.abstractAlthough vitamin D (VD) is known to have multiple effects on the skin and immunity, its effects on atopic dermatitis (AD) severity remain unclear. We investigated whether oral cholecalciferol (VD3) supplementation changes stratum corneum expression of the vitamin D receptor (vdr), and the epidermal alarmins Cathelicidin Antimicrobial Peptide (camp/LL-37) and Thymic Stromal Lymphopoietin (tslp) in children with AD. We conducted an open-label supplementation study with weekly oral VD3 for six weeks in children with AD. Serum 25-hydroxyvitamin D (25OHD), lesional Staphylococcus aureus colonization, and AD severity evaluated by SCORAD index were evaluated before and after supplementation. Tape stripping (TS) was performed on non-lesional and lesional skin to measure mRNA expression of vdr, camp, and tslp through RT-qPCR and LL-37 peptide by ELISA. Twenty-two children with moderate–severe AD received weekly oral VD3 for six weeks. Total serum 25OHD increased from 45.1 ± 23 to 93.5 ± 24.3 nmoL/L (p < 0.0001), while SCORAD decreased from 41.4 ± 13.5 to 31.5 ± 15.8 (p < 0.0001). After treatment, epidermal gene expression of camp increased significantly in non-lesional (p = 0.014) and lesional (p = 0.0007) tape stripping samples, while vdr only increased in lesional skin samples (p < 0.0001). LL-37 peptide increased significantly only in lesional skin samples (p = 0.008). Gene expression of tslp did not change after oral VD3 treatment. In children with AD, oral VD3 supplementation was associated with improved VD status and AD severity, as well as increased VDR and Cathelicidin expression in lesional skin, which provide mechanistic clues on its effects.
dc.fechaingreso.objetodigital2023-01-19
dc.format.extent10 páginas
dc.fuente.origenSIPA
dc.identifier.doi10.1007/s00403-022-02416-1
dc.identifier.eissn1432-069X
dc.identifier.urihttps://doi.org/10.1007/s00403-022-02416-1
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/66377
dc.information.autorucFacultad de medicina ; Borzutzky Schachter, Arturo ; 0000-0002-7904-262X ; 5897
dc.language.isoen
dc.nota.accesoContenido completo
dc.revistaArchives of Dermatological Research
dc.rightsacceso abierto
dc.subjectAlarmins
dc.subjectVdr
dc.subjectCathelicidin
dc.subjectTSLP
dc.subjectStaphylococcus aureus
dc.subjectVitamin D
dc.subjectAtopic dermatitis
dc.subjectEczema
dc.titleOral vitamin D modulates the epidermal expression of the vitamin D receptor and cathelicidin in children with atopic dermatitis
dc.typeartículo
sipa.codpersvinculados5897
sipa.indexSCOPUS
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