Meristem culture and subsequent micropropagation of chilean strawberry (Fragaria chiloensis (L.) Duch.)

dc.contributor.authorQuiroz, Karla A.
dc.contributor.authorCarrasco Gálvez, Basilio Alejandro
dc.contributor.authorBerríos, Miguel.
dc.contributor.authorRetamales, Jorge B.
dc.contributor.authorCaligari, Peter D. S.
dc.contributor.authorGarcía-Gonzáles, Rolando.
dc.date.accessioned2019-10-17T14:43:41Z
dc.date.available2019-10-17T14:43:41Z
dc.date.issued2017
dc.date.updated2019-10-14T19:12:09Z
dc.description.abstractAbstract Background Vegetative propagation of Fragaria sp. is traditionally carried out using stolons. This system of propagation, in addition to being slow, can spread plant diseases, particularly serious being viral. In vitro culture of meristems and the establishment of micropropagation protocols are important tools for solving these problems. In recent years, considerable effort has been made to develop in vitro propagation of the commercial strawberry in order to produce virus-free plants of high quality. These previous results can serve as the basis for developing in vitro-based propagation technologies in the less studied species Fragaria chiloensis. Results In this context, we studied the cultivation of meristems and establishment of a micropropagation protocol for F. chiloensis. The addition of polyvinylpyrrolidone (PVP) improved the meristem regeneration efficiency of F. chiloensis accessions. Similarly, the use of 6-benzylaminopurine (BAP) in the culture media increased the average rate of multiplication to 3–6 shoots per plant. In addition, the use of 6-benzylaminopurine (BAP), had low levels (near zero) of explant losses due to oxidation. However, plant height as well as number of leaves and roots were higher in media without growth regulators, with average values of 0.5 cm, 9 leaves and 4 roots per plant. Conclusions For the first time in Chilean strawberry, meristem culture demonstrated to be an efficient tool for eliminating virus from infected plants, giving the possibility to produce disease free propagation material. Also, the addition of PVP into the basal MS medium improved the efficiency of plant recovery from isolated meristems. Farmers can now access to high quality plant material produced by biotech tools which will improve their technological practices.Abstract Background Vegetative propagation of Fragaria sp. is traditionally carried out using stolons. This system of propagation, in addition to being slow, can spread plant diseases, particularly serious being viral. In vitro culture of meristems and the establishment of micropropagation protocols are important tools for solving these problems. In recent years, considerable effort has been made to develop in vitro propagation of the commercial strawberry in order to produce virus-free plants of high quality. These previous results can serve as the basis for developing in vitro-based propagation technologies in the less studied species Fragaria chiloensis. Results In this context, we studied the cultivation of meristems and establishment of a micropropagation protocol for F. chiloensis. The addition of polyvinylpyrrolidone (PVP) improved the meristem regeneration efficiency of F. chiloensis accessions. Similarly, the use of 6-benzylaminopurine (BAP) in the culture media increased the average rate of multiplication to 3–6 shoots per plant. In addition, the use of 6-benzylaminopurine (BAP), had low levels (near zero) of explant losses due to oxidation. However, plant height as well as number of leaves and roots were higher in media without growth regulators, with average values of 0.5 cm, 9 leaves and 4 roots per plant. Conclusions For the first time in Chilean strawberry, meristem culture demonstrated to be an efficient tool for eliminating virus from infected plants, giving the possibility to produce disease free propagation material. Also, the addition of PVP into the basal MS medium improved the efficiency of plant recovery from isolated meristems. Farmers can now access to high quality plant material produced by biotech tools which will improve their technological practices.
dc.fuente.origenBiomed Central
dc.identifier.citationBiological Research. 2017 Jun 02;50(1):20
dc.identifier.doi10.1186/s40659-017-0125-8
dc.identifier.urihttps://dx.doi.org/10.1186/s40659-017-0125-8
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/26725
dc.issue.numeroNo. 20
dc.language.isoen
dc.pagina.final11
dc.pagina.inicio1
dc.revistaBiological Researches_ES
dc.rightsacceso abierto
dc.rights.holderThe Author(s)
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.otherFragaria chiloensises_ES
dc.subject.otherPlantas - Crecimientoes_ES
dc.subject.otherPlantas viruses_ES
dc.titleMeristem culture and subsequent micropropagation of chilean strawberry (Fragaria chiloensis (L.) Duch.)es_ES
dc.typeartículo
dc.volumenVol. 50
sipa.codpersvinculados174497
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