Isolation and engineering of a Listeria grayi bacteriophage

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dc.contributor.authorErickson, Stephen
dc.contributor.authorPaulson, John
dc.contributor.authorBrown, Matthew
dc.contributor.authorHahn, Wendy
dc.contributor.authorGil, Jose
dc.contributor.authorBarron Montenegro, Rocio
dc.contributor.authorMoreno Switt, Andrea, I
dc.contributor.authorEisenberg, Marcia
dc.contributor.authorNguyen, Minh M.
dc.date.accessioned2024-01-10T13:44:51Z
dc.date.available2024-01-10T13:44:51Z
dc.date.issued2021
dc.description.abstractThe lack of bacteriophages capable of infecting the Listeria species, Listeria grayi, is academically intriguing and presents an obstacle to the development of bacteriophage-based technologies for Listeria. We describe the isolation and engineering of a novel L. grayi bacteriophage, LPJP1, isolated from farm silage. With a genome over 200,000 base pairs, LPJP1 is the first and only reported jumbo bacteriophage infecting the Listeria genus. Similar to other Gram-positive jumbo phages, LPJP1 appeared to contain modified base pairs, which complicated initial attempts to obtain genomic sequence using standard methods. Following successful sequencing with a modified approach, a recombinant of LPJP1 encoding the NanoLuc luciferase was engineered using homologous recombination. This luciferase reporter bacteriophage successfully detected 100 stationary phase colony forming units of both subspecies of L. grayi in four hours. A single log phase colony forming unit was also sufficient for positive detection in the same time period. The recombinant demonstrated complete specificity for this particular Listeria species and did not infect 150 non-L. grayi Listeria strains nor any other bacterial genus. LPJP1 is believed to be the first reported lytic bacteriophage of L. grayi as well as the only jumbo bacteriophage to be successfully engineered into a luciferase reporter.
dc.fechaingreso.objetodigital2024-04-25
dc.format.extent12 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1038/s41598-021-98134-1
dc.identifier.issn2045-2322
dc.identifier.pubmedidMEDLINE:34556683
dc.identifier.urihttps://doi.org/10.1038/s41598-021-98134-1
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/78945
dc.identifier.wosidWOS:000698791600101
dc.information.autorucFacultad de Agronomía e Ingeniería Forestal; Moreno Switt, Andrea Isabel; S/I; 1147061
dc.issue.numero1
dc.language.isoen
dc.nota.accesocontenido completo
dc.publisherNATURE PORTFOLIO
dc.revistaSCIENTIFIC REPORTS
dc.rightsacceso abierto
dc.subjectLUCIFERASE REPORTER
dc.subjectPHAGE A511
dc.subjectDNA
dc.subjectGENOME
dc.subjectSPP.
dc.subjectMONOCYTOGENES
dc.subjectBACTEREMIA
dc.subjectFOOD
dc.subjectSUBSTITUTION
dc.subjectENVIRONMENT
dc.titleIsolation and engineering of a Listeria grayi bacteriophage
dc.typeartículo
dc.volumen11
sipa.codpersvinculados1147061
sipa.indexWOS
sipa.trazabilidadCarga SIPA;09-01-2024
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