Calcium (Ca2+) waves data calibration and analysis using image processing techniques

dc.contributor.authorMilovic Fabregat, Carlos Andrés
dc.contributor.authorOses, Carolina
dc.contributor.authorVillalón, Manuel J.
dc.contributor.authorUribe Arancibia, Sergio A.
dc.contributor.authorPrieto Vásquez, Claudia
dc.contributor.authorAndía Kohnenkampf, Marcelo Edgardo
dc.contributor.authorIrarrázaval Mena, Pablo
dc.contributor.authorTejos Núñez, Cristián Andrés
dc.contributor.authorLizama, Carlos
dc.date.accessioned2019-10-17T13:16:07Z
dc.date.available2019-10-17T13:16:07Z
dc.date.issued2013
dc.date.updated2019-10-14T18:27:35Z
dc.description.abstractAbstract Background Calcium (Ca2+) propagates within tissues serving as an important information carrier. In particular, cilia beat frequency in oviduct cells is partially regulated by Ca2+ changes. Thus, measuring the calcium density and characterizing the traveling wave plays a key role in understanding biological phenomena. However, current methods to measure propagation velocities and other wave characteristics involve several manual or time-consuming procedures. This limits the amount of information that can be extracted, and the statistical quality of the analysis. Results Our work provides a framework based on image processing procedures that enables a fast, automatic and robust characterization of data from two-filter fluorescence Ca2+ experiments. We calculate the mean velocity of the wave-front, and use theoretical models to extract meaningful parameters like wave amplitude, decay rate and time of excitation. Conclusions Measurements done by different operators showed a high degree of reproducibility. This framework is also extended to a single filter fluorescence experiments, allowing higher sampling rates, and thus an increased accuracy in velocity measurements.
dc.fuente.origenBiomed Central
dc.identifier.citationBMC Bioinformatics. 2013 May 16;14(1):162
dc.identifier.doi10.1186/1471-2105-14-162
dc.identifier.urihttps://doi.org/10.1186/1471-2105-14-162
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/26614
dc.issue.numeroNo. 162
dc.language.isoen
dc.nota.accesoContenido completo
dc.pagina.final14
dc.pagina.inicio1
dc.revistaBMC Bioinformaticses_ES
dc.rightsacceso abierto
dc.rights.holderMilovic et al.; licensee BioMed Central Ltd.
dc.subject.ddc570
dc.subject.deweyBiologíaes_ES
dc.subject.otherCalcio - Fisiologíaes_ES
dc.subject.otherCalcio - Investigacioneses_ES
dc.subject.otherOviductoses_ES
dc.titleCalcium (Ca2+) waves data calibration and analysis using image processing techniqueses_ES
dc.typeartículo
dc.volumenVol. 14
sipa.codpersvinculados99264
sipa.codpersvinculados14195
sipa.codpersvinculados90691
sipa.codpersvinculados57376
sipa.codpersvinculados4027
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