Calcium (Ca2+) waves data calibration and analysis using image processing techniques

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dc.contributor.authorMilovic Fabregat, Carlos Andrés
dc.contributor.authorOses, Carolina
dc.contributor.authorVillalón, Manuel J.
dc.contributor.authorUribe Arancibia, Sergio A.
dc.contributor.authorPrieto Vásquez, Claudia
dc.contributor.authorAndía Kohnenkampf, Marcelo Edgardo
dc.contributor.authorIrarrázaval Mena, Pablo
dc.contributor.authorTejos Núñez, Cristián Andrés
dc.contributor.authorLizama, Carlos
dc.date.accessioned2019-10-17T13:16:07Z
dc.date.available2019-10-17T13:16:07Z
dc.date.issued2013
dc.date.updated2019-10-14T18:27:35Z
dc.description.abstractAbstract Background Calcium (Ca2+) propagates within tissues serving as an important information carrier. In particular, cilia beat frequency in oviduct cells is partially regulated by Ca2+ changes. Thus, measuring the calcium density and characterizing the traveling wave plays a key role in understanding biological phenomena. However, current methods to measure propagation velocities and other wave characteristics involve several manual or time-consuming procedures. This limits the amount of information that can be extracted, and the statistical quality of the analysis. Results Our work provides a framework based on image processing procedures that enables a fast, automatic and robust characterization of data from two-filter fluorescence Ca2+ experiments. We calculate the mean velocity of the wave-front, and use theoretical models to extract meaningful parameters like wave amplitude, decay rate and time of excitation. Conclusions Measurements done by different operators showed a high degree of reproducibility. This framework is also extended to a single filter fluorescence experiments, allowing higher sampling rates, and thus an increased accuracy in velocity measurements.
dc.fuente.origenBiomed Central
dc.identifier.citationBMC Bioinformatics. 2013 May 16;14(1):162
dc.identifier.doi10.1186/1471-2105-14-162
dc.identifier.urihttps://doi.org/10.1186/1471-2105-14-162
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/26614
dc.issue.numeroNo. 162
dc.language.isoen
dc.nota.accesoContenido completo
dc.pagina.final14
dc.pagina.inicio1
dc.revistaBMC Bioinformaticses_ES
dc.rightsacceso abierto
dc.rights.holderMilovic et al.; licensee BioMed Central Ltd.
dc.subject.ddc570
dc.subject.deweyBiologíaes_ES
dc.subject.otherCalcio - Fisiologíaes_ES
dc.subject.otherCalcio - Investigacioneses_ES
dc.subject.otherOviductoses_ES
dc.titleCalcium (Ca2+) waves data calibration and analysis using image processing techniqueses_ES
dc.typeartículo
dc.volumenVol. 14
sipa.codpersvinculados99264
sipa.codpersvinculados14195
sipa.codpersvinculados90691
sipa.codpersvinculados57376
sipa.codpersvinculados4027
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