Functional and Structural Analysis of the Internal Ribosome Entry Site Present in the mRNA of Natural Variants of the HIV-1

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dc.contributor.authorVallejos, Maricarmen
dc.contributor.authorCarvajal, Felipe
dc.contributor.authorPino, Karla
dc.contributor.authorNavarrete, Camilo
dc.contributor.authorFerres, Marcela
dc.contributor.authorPablo Huidobro Toro, Juan
dc.contributor.authorSargueil, Bruno
dc.contributor.authorLopez Lastra, Marcelo
dc.date.accessioned2024-01-10T13:13:13Z
dc.date.available2024-01-10T13:13:13Z
dc.date.issued2012
dc.description.abstractThe 5'untranslated regions (UTR) of the full length mRNA of the HIV-1 proviral clones pNL4.3 and pLAI, harbor an internal ribosomal entry site (IRES). In this study we extend this finding by demonstrating that the mRNA 5'UTRs of natural variants of HIV-1 also exhibit IRES-activity. Cap-independent translational activity was demonstrated using bicistronic mRNAs in HeLa cells and in Xenopus laevis oocytes. The possibility that expression of the downstream cistron in these constructs was due to alternative splicing or to cryptic promoter activity was ruled out. The HIV-1 variants exhibited significant 5'UTR nucleotide diversity with respect to the control sequence recovered from pNL4.3. Interestingly, translational activity from the 5'UTR of some of the HIV-1 variants was enhanced relative to that observed for the 5'UTR of pNL4.3. In an attempt to explain these findings we probed the secondary structure of the variant HIV-1 5'UTRs using enzymatic and chemical approaches. Yet subsequent structural analyses did not reveal significant variations when compared to the pNL4.3-5'UTR. Thus, the increased IRES-activity observed for some of the HIV-1 variants cannot be ascribed to a specific structural modification. A model to explain these findings is proposed.
dc.description.funderFondo Nacional de Ciencia y Tecnologia del Gobierno de Chile (FONDECYT)
dc.description.funderProyecto
dc.description.funderde la Iniciativa Cientifica Milenio del Ministerio de Economia
dc.description.funderFomento y Turismo
dc.description.funderSIDACTION
dc.description.funderAgence Nationale de Recherche sur le Sida (ANRS)
dc.description.funderCentre National de la Recherche Scientifique (CNRS)-France
dc.description.funderComision Nacional de Investigacion Cientofica y Tecnologica (CONICYT)-Chile
dc.description.funderPontificia Universidad Catolica de Chile
dc.description.funderCONICYT
dc.format.extent12 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1371/journal.pone.0035031
dc.identifier.issn1932-6203
dc.identifier.pubmedidMEDLINE:22496887
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0035031
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/78280
dc.identifier.wosidWOS:000304855200096
dc.information.autorucCiencias Biológicas;Carvajal F;S/I;171996
dc.information.autorucMedicina;Ferres M;S/I;66180
dc.information.autorucMedicina;López-Lastra M;S/I;84823
dc.information.autorucCiencias Biológicas;Navarrete C;S/I;163596
dc.information.autorucMedicina;Pino K;S/I;1005654
dc.information.autorucMedicina;Vallejos M;S/I;132558
dc.issue.numero4
dc.language.isoen
dc.nota.accesoSin adjunto
dc.publisherPUBLIC LIBRARY SCIENCE
dc.revistaPLOS ONE
dc.rightsregistro bibliográfico
dc.subjectCAP-INDEPENDENT TRANSLATION
dc.subjectPROTEIN-SYNTHESIS
dc.subject5-UNTRANSLATED REGION
dc.subjectPROMOTES TRANSLATION
dc.subjectVIRUS LEADER
dc.subjectINITIATION
dc.subjectIRES
dc.subjectSEGMENT
dc.subjectEXPRESSION
dc.subjectMECHANISM
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleFunctional and Structural Analysis of the Internal Ribosome Entry Site Present in the mRNA of Natural Variants of the HIV-1
dc.typeartículo
dc.volumen7
sipa.codpersvinculados171996
sipa.codpersvinculados66180
sipa.codpersvinculados84823
sipa.codpersvinculados163596
sipa.codpersvinculados1005654
sipa.codpersvinculados132558
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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