A high throughput method for detection of cyclooxygenase-2 enzyme inhibitors by effect-directed analysis applying high performance thin layer chromatography-bioassay-mass spectrometry

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dc.catalogadorgrr
dc.contributor.authorOyarzún, Paulina
dc.contributor.authorCarrasco, Jonathan
dc.contributor.authorPeterssen, Darlene
dc.contributor.authorTereucan, Gonzalo
dc.contributor.authorAranda, Mario
dc.contributor.authorHenriquez-Aedo, Karem
dc.date.accessioned2023-12-14T18:53:42Z
dc.date.available2023-12-14T18:53:42Z
dc.date.issued2023
dc.description.abstractA high throughput method was developed to detect bioactive molecules with inhibitory activity over cyclo-oxygenase (COX-2) enzyme applying effect-directed analysis and planar chromatography hyphenated with bioassay and mass spectrometry. The assay was based on the indirect measurement of arachidonic acid trans-formation into prostaglandin with the colorimetric co-substrate N,N,N',N'-tetramethyl-p-phenylenediamine. Inhibitory zones were observed as colorless bands over a blue background. Using a central composite design the critical factors like substrate concentration, enzyme: substrate ratio, reaction time, and co-substrate concentra-tion were optimized. Optimal conditions were achieved with 0.03 mg/mL of arachidonic acid, 0.15 U/mL of COX-2, and 8.21 mg/mL of chromogenic reagent. Method usefulness was challenged analyzing fresh Chiloe's giant garlic (Allium ampeloprasum L) ethanol: water (8:2 v/v) extract, finding COX-2 inhibitors that were pre-liminarily identified as the isomers gamma-glutamyl-S-allyl-L-cysteine and gamma-glutamyl-S-(trans-1-propenyl)-L-cysteine.
dc.fechaingreso.objetodigital2023-12-14
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.chroma.2023.464426
dc.identifier.eissn1873-3778
dc.identifier.issn0021-9673
dc.identifier.pubmedid37862751
dc.identifier.scopusidSCOPUS_ID: 85174177623
dc.identifier.urihttps://doi.org/10.1016/j.chroma.2023.464426
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/75495
dc.identifier.wosidWOS:001097546500001
dc.information.autorucEscuela de Química y Farmacia; Oyarzún, Paulina ; 0009-0008-9405-3870; 1298888
dc.languageen
dc.language.isoen
dc.nota.accesoContenido parcial
dc.publisherElsevier
dc.revistaJournal of Chromatography A
dc.rightsacceso restringido
dc.subjectAnti-inflammatory
dc.subjectTmpd Mass spectrometry
dc.subjectAllium ampeloprasum l
dc.subjectCOX and HPTLC
dc.subject.ddc510
dc.subject.deweyMatemática física y químicaes_ES
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleA high throughput method for detection of cyclooxygenase-2 enzyme inhibitors by effect-directed analysis applying high performance thin layer chromatography-bioassay-mass spectrometry
dc.typeartículo
dc.volumen1711
sipa.codpersvinculados1298888
sipa.trazabilidadWOS;2023-11-25
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