The immunophenotype of amniotic fluid leukocytes in normal and complicated pregnancies

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dc.contributor.authorGomez Lopez, Nardhy
dc.contributor.authorRomero, Roberto
dc.contributor.authorXu, Yi
dc.contributor.authorMiller, Derek
dc.contributor.authorLeng, Yaozhu
dc.contributor.authorPanaitescu, Bogdan
dc.contributor.authorSilva, Pablo
dc.contributor.authorFaro, Jonathan
dc.contributor.authorAlhousseini, Ali
dc.contributor.authorGill, Navleen
dc.contributor.authorHassan, Sonia S.
dc.contributor.authorHsu, Chaur Dong
dc.date.accessioned2024-01-10T13:43:50Z
dc.date.available2024-01-10T13:43:50Z
dc.date.issued2018
dc.description.abstractProblemThe immune cellular composition of amniotic fluid is poorly understood. Herein, we determined: 1) the immunophenotype of amniotic fluid immune cells during the second and third trimester in the absence of intra-amniotic infection/inflammation; 2) whether amniotic fluid T cells and ILCs display different phenotypical characteristics to that of peripheral cells; and 3) whether the amniotic fluid immune cells are altered in women with intra-amniotic infection/inflammation.
dc.description.abstractMethod of StudyAmniotic fluid samples (n=57) were collected from 15 to 40weeks of gestation in women without intra-amniotic infection/inflammation. Samples from women with intra-amniotic infection/inflammation were also included (n=9). Peripheral blood mononuclear cells from healthy adults were used as controls (n=3). Immunophenotyping was performed using flow cytometry.
dc.description.abstractResultsIn the absence of intra-amniotic infection/inflammation, the amniotic fluid contained several immune cell populations between 15 and 40 weeks. Among these immune cells: (i) T cells and ILCs were greater than B cells and natural killer (NK) cells between 15 and 30weeks; (ii) T cells were most abundant between 15 and 30weeks; (iii) ILCs were most abundant between 15 and 20weeks; (iv) B cells were scarce between 15 and 20weeks; yet, they increased and were constant after 20weeks; (v) NK cells were greater between 15 and 30weeks than at term; (vi) ILCs expressed high levels of RORt, CD161, and CD103 (ie, group 3 ILCs); (vii) T cells expressed high levels of RORt; (viii) neutrophils increased as gestation progressed; and (ix) monocytes/macrophages emerged after 20weeks and remained constant until term. All of the amniotic fluid immune cells, except ILCs, were increased in the presence of intra-amniotic infection/inflammation.
dc.description.abstractConclusionThe amniotic fluid harbors a diverse immune cellular composition during normal and complicated pregnancies.
dc.description.funderPerinatology Research Branch (PRB), Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, U.S. Department of Health and Human Services (NICHD/NIH/DHHS)
dc.description.funderNICHD/NIH/DHHS
dc.description.funderWayne State University Perinatal Initiative in Maternal, Perinatal, and Child Health
dc.description.funderEUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT
dc.description.funderEUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH &HUMAN DEVELOPMENT
dc.fechaingreso.objetodigital2024-05-28
dc.format.extent17 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1111/aji.12827
dc.identifier.eissn1600-0897
dc.identifier.issn1046-7408
dc.identifier.pubmedidMEDLINE:29500850
dc.identifier.urihttps://doi.org/10.1111/aji.12827
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/78767
dc.identifier.wosidWOS:000428349300008
dc.information.autorucCiencias de la Salud; Silva P ;S/I;1067208
dc.issue.numero4
dc.language.isoen
dc.nota.accesocontenido parcial
dc.publisherWILEY
dc.revistaAMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
dc.rightsacceso restringido
dc.subjectB cells
dc.subjectbacteria
dc.subjectfetal immunity
dc.subjectimmune cells
dc.subjectinnate lymphoid cells
dc.subjectintra-amniotic infection
dc.subjectintra-amniotic inflammation
dc.subjectleukocytes
dc.subjectmacrophages
dc.subjectmicrobes
dc.subjectmicrobial invasion of the amniotic cavity
dc.subjectmonocytes
dc.subjectmucosal immunity
dc.subjectneutrophils
dc.subjectnatural killer (NK) cells
dc.subjectT cells
dc.subjectBLOOD-CELL COUNT
dc.subjectINNATE LYMPHOID-CELLS
dc.subjectTUMOR-NECROSIS-FACTOR
dc.subjectACTIVATING PEPTIDE-1 INTERLEUKIN-8
dc.subjectBACTERIAL-GROWTH INHIBITION
dc.subjectPRETERM PREMATURE RUPTURE
dc.subjectREGULATORY T-CELLS
dc.subjectMICROBIAL INVASION
dc.subjectINTRAAMNIOTIC INFLAMMATION
dc.subjectCLINICAL-SIGNIFICANCE
dc.subject.ods05 Gender Equality
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa05 Igualdad de género
dc.subject.odspa03 Salud y bienestar
dc.titleThe immunophenotype of amniotic fluid leukocytes in normal and complicated pregnancies
dc.typeartículo
dc.volumen79
sipa.codpersvinculados1067208
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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