D-Glucose stimulation of L-arginine transport and nitric oxicle synthesis results from activation of mitogen-activated protein kinases p42/44 and smad2 requiring functional type II TGF-beta receptors in human umbilical vein endothelium

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dc.contributor.authorVasquez, Rodrigo
dc.contributor.authorFarias, Marcelo
dc.contributor.authorVega, Jost Luis
dc.contributor.authorMartin, Rody San
dc.contributor.authorVecchiola, Andrea
dc.contributor.authorCasanello, Paola
dc.contributor.authorSobrevia, Luis
dc.date.accessioned2024-01-10T13:12:18Z
dc.date.available2024-01-10T13:12:18Z
dc.date.issued2007
dc.description.abstractElevated extracellular D-glucose increases transforming growth factor P I (TGF-P 1) release from human umbilical vein endothelium (HUVEC). TGF-P 1, via TGF-P receptors I (T beta RI) and T beta RII, activates Smad2 and mitogen -activated protein kinases p44 and p42 (p42/44 (mapk)). We studied whether D-glucose-stimulation Of L-arginine transport and nitric oxide synthesis involves TGF-beta 1 in primary cultures of HUVEC. TGF-P I release was higher (similar to 1.6-fold) in 25 mM (high) compared with 5 mM (normal) D-glucose. TGF-P I increases L-arginine transport (half maximal effect similar to 1.6 ng/ml) in normal D-glucose, but did not alter high D-glucose-increased L-arginine transport. TGF-P I and high D-glucose increased hCAT- I mRNA expression (similar to 8-fold) and maximal transport velocity (V-max), L- [(3) H]citrulline formation from L- [3 H]arginine (index of NO synthesis) and endothelial NO synthase (eNOS) protein abundance, but did not alter eNOS phosphorylation. TGF-beta 1 I and high D-gludose increased p42/44 mapk and Smad2 phosphorylation, an effect blocked by PD-98059 (MEK 1 /2 inhibitor). However, TGF-P I and high D-glucose were ineffective in cells expressing a truncated, negative dominant T beta RII High D-glucose increases L-arginine transport and eNOS expression following T beta RII activation by TGF-P I involving p42/44 (mapk) and Smad2 in HUVEC. Thus, TGF-P I could play a crucial role under conditions of hyperglycemia, such as gestational diabetes mellitus, which is
dc.format.extent7 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1002/jcp.21057
dc.identifier.eissn1097-4652
dc.identifier.issn0021-9541
dc.identifier.pubmedidMEDLINE:17427197
dc.identifier.urihttps://doi.org/10.1002/jcp.21057
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/78167
dc.identifier.wosidWOS:000248770500009
dc.information.autorucMedicina;Casanello P;S/I;146772
dc.information.autorucMedicina;Farías M;S/I;12286
dc.information.autorucMedicina;San Martin R;S/I;1003218
dc.information.autorucMedicina;Sobrevia L;S/I;1002656
dc.information.autorucCiencias Biológicas;Vecchiola A;S/I;123319
dc.information.autorucCiencias Biológicas;Vega J;S/I;165832
dc.information.autorucCiencias Biológicas;Vásquez R;S/I;6
dc.issue.numero3
dc.language.isoen
dc.nota.accesoSin adjunto
dc.pagina.final632
dc.pagina.inicio626
dc.publisherWILEY
dc.revistaJOURNAL OF CELLULAR PHYSIOLOGY
dc.rightsregistro bibliográfico
dc.subjectGROWTH-FACTOR-BETA
dc.subjectSMOOTH-MUSCLE-CELLS
dc.subjectOXIDE SYNTHASE EXPRESSION
dc.subjectSIGNAL-REGULATED KINASE
dc.subjectHUMAN MESANGIAL CELLS
dc.subjectAMINO-ACID
dc.subjectACUTE HYPERGLYCEMIA
dc.subjectVASODILATION
dc.subjectDYSFUNCTION
dc.subjectTGF-BETA-1
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleD-Glucose stimulation of L-arginine transport and nitric oxicle synthesis results from activation of mitogen-activated protein kinases p42/44 and smad2 requiring functional type II TGF-beta receptors in human umbilical vein endothelium
dc.typeartículo
dc.volumen212
sipa.codpersvinculados146772
sipa.codpersvinculados12286
sipa.codpersvinculados1003218
sipa.codpersvinculados1002656
sipa.codpersvinculados123319
sipa.codpersvinculados165832
sipa.codpersvinculados6
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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