Uptake of dodecanedioic acid by isolated rat liver

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dc.contributor.authorGreco, AV
dc.contributor.authorMingrone, G
dc.contributor.authorDeGaetano, A
dc.contributor.authorAmigo, L
dc.contributor.authorPuglielli, L
dc.contributor.authorCastagneto, M
dc.contributor.authorNervi, F
dc.date.accessioned2024-01-10T13:44:06Z
dc.date.available2024-01-10T13:44:06Z
dc.date.issued1997
dc.description.abstractThe uptake of dodecanedioic acid (C12), a dicarboxylic acid with 12 carbon atoms, was studied in the isolated perfused rat liver. Fifty pmol of C12 were injected as a bolus into the perfusing liver solution. The concentration of C12 in perfusate samples taken over 2 h from the beginning bf the experiments were analyzed by high performance liquid chromatography. An in vitro experimental session was performed to determine the binding curve of C12 to defatted bovine serum albumin. These data were then used to compute the perfusate C12 free fraction. The number of binding sites on the albumin molecule was equal to 4.29 +/- 0.21 (S.E.), while the affinity constant was 6.33 +/- 0.87 x 10(3) M(-1). Experimental values of perfusate C12 concentration versus time were individually plotted and fitted to a monoexponential decay for each liver perfused. The predicted C12 concentration at time zero averaged 0.354 +/- 0.0375 mu mol/ml. From this value the apparent volume of distribution of C12 was obtained and corresponded to 153.02 +/- 14.56 mi. The disappearance rate constant from the perfusate was 0.0278 +/- 0.0030 min(-1). The C12 half life was 26.6 +/- 2.3 min. The mean hepatic clearance from the perfusate was 4.08 +/- 0.38 ml/min. In conclusion, C12 is quickly taken up by the liver so that in about 100 min it was completely cleared from the perfusate. (C) 1997 Elsevier Science B.V.
dc.fechaingreso.objetodigital16-04-2024
dc.format.extent10 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1016/S0009-8981(96)06457-1
dc.identifier.issn0009-8981
dc.identifier.pubmedidMEDLINE:9074817
dc.identifier.urihttps://doi.org/10.1016/S0009-8981(96)06457-1
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/78838
dc.identifier.wosidWOS:A1997WK65400008
dc.information.autorucMedicina;Nervi F;S/I;99156
dc.issue.numero2
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.final218
dc.pagina.inicio209
dc.publisherELSEVIER SCIENCE BV
dc.revistaCLINICA CHIMICA ACTA
dc.rightsacceso restringido
dc.subjectdodecanedioic acid
dc.subjectdicarboxylic acid
dc.subjectHPLC
dc.subjectisolated liver perfusion
dc.subjectmathematical models
dc.subjectPEROXISOMAL FATTY-ACID
dc.subjectHUMAN PLASMA-ALBUMIN
dc.subjectDICARBOXYLIC-ACIDS
dc.subjectFUEL SUBSTRATE
dc.subjectAZELAIC ACID
dc.subjectPHARMACOKINETIC ANALYSIS
dc.subjectPARENTERAL-NUTRITION
dc.subjectDISODIUM SEBACATE
dc.subjectBETA-OXIDATION
dc.subjectHEPATOCYTES
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleUptake of dodecanedioic acid by isolated rat liver
dc.typeartículo
dc.volumen258
sipa.codpersvinculados99156
sipa.indexWOS
sipa.trazabilidadCarga SIPA;09-01-2024
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