Time-resolved fluorescence and anisotropy-sensitive 1,2-dimyristoyl-sn-glycero-3-(7-aminocoumarin) phosphoetanolamide probe for studying membrane lipid domains

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dc.catalogadorpau
dc.contributor.authorZúñiga Núñez, Daniel
dc.contributor.authorMura, Francisco
dc.contributor.authorMariño-Ocampo, Nory
dc.contributor.authorBriones-Rebolledo, Patricio
dc.contributor.authorPoblete, Horacio
dc.contributor.authorMallet, Jean-Maurice
dc.contributor.authorFuentealba Patino, Denis Alberto
dc.contributor.authorAspee, Alexis
dc.date.accessioned2023-08-04T21:20:28Z
dc.date.available2023-08-04T21:20:28Z
dc.date.issued2023
dc.description.abstractA fluorescent probe C-DMPE was synthesised to monitor interfacial membrane properties by conjugating coumarin-343 and 1,2-dimyristoyl-sn-glycero-3-phosphorylethanolamine (DMPE), anchoring the 7-aminocou-marin moiety close to the phospholipid polar head at the membrane interface. Large unilamellar vesicles (LUV) of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC), of 1,2-dioleyl-sn-glycero-3-phosphatidyl-choline (DOPC) and cholesterol were employed as a model of lipid bilayer. Time-resolved fluorescence developed an emissive Internal Charge Transfer excited state with a long fluorescence lifetime (T1), a Locally Excited state with an intermediate fluorescence lifetime (T2), and a short lifetime (T3) associated with an intermolecular quenching by interaction with a phosphate group of neighbour phospholipids, as is clearly shown by molecular dynamics simulations. Shorter values of fluorescence lifetimes T1 and T3 were observed in DOPC with respect to DPPC, responding to a more fluid membrane with more significant water accessibility in DOPC than DPPC. However, in DPPC:DOPC vesicles, these fluorescence lifetimes are even shorter, allowing to be attributed to favourable sensing of boundary limit lipid domains. In similitude, time-resolved anisotropy showed shorter rotational correlation times phi 1, in DPPC: DOPC vesicles than in DOPC associated with a faster internal rotational movement of the 7-aminocoumarin group in domains than in fluid a DOPC membrane. In addition, shorter rotational correlation times, (P2, were also observed in DPPC:DOPC vesicles compared to DPPC, suggesting a faster lateral diffusion of the probe in the presence of domains.
dc.fechaingreso.objetodigital2023-07-04
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.dyepig.2023.111368
dc.identifier.eissn1873-3743
dc.identifier.issn0143-7208
dc.identifier.urihttps://doi.org/10.1016/j.dyepig.2023.111368
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/74353
dc.identifier.wosidWOS:001009414600001
dc.information.autorucEscuela de Química; Mariño-Ocampo, Nory; ; 1059618
dc.information.autorucEscuela de Química; Fuentealba Patino, Denis Alberto; 0000-0003-4798-7204; 160255
dc.language.isoen
dc.nota.accesoContenido completo
dc.publisherELSEVIER SCI LTD
dc.revistaDYES AND PIGMENTS
dc.rightsacceso abierto
dc.subjectFluorescence
dc.subjectAnisotropy
dc.subjectDPPC
dc.subjectDOPC
dc.subjectLUVs
dc.subjectLiposomes
dc.subject.ddc510
dc.subject.deweyMatemática física y químicaes_ES
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleTime-resolved fluorescence and anisotropy-sensitive 1,2-dimyristoyl-sn-glycero-3-(7-aminocoumarin) phosphoetanolamide probe for studying membrane lipid domains
dc.typeartículo
dc.volumen217
sipa.codpersvinculados1059618
sipa.codpersvinculados160255
sipa.indexWOS
sipa.trazabilidadWOS;2023-07-06
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