<i>In vitro</i> protein breakdown by enzyme extracts of rumen origin: comparison with methods <i>in situ</i> and proteases of <i>Streptomyces griseus</i>

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dc.contributor.authorVelasquez, Alejandro
dc.contributor.authorPichard, Gaston
dc.date.accessioned2025-01-21T00:04:53Z
dc.date.available2025-01-21T00:04:53Z
dc.date.issued2010
dc.description.abstractA. Velasquez, and G. Pichard. 2010. In vitro protein breakdown by enzyme extracts of rumen origin: comparison with methods in situ and proteases of Streptomyces griseus. Cien. Inv. Agr. 37(3): 57-70. Proteolytic activity of enzymatic extracts generated from rumen microorganisms cultivated in vitro was evaluated. The incubation of rumen fluid used different substrates to generate a higher enzyme concentration and promote a broad spectrum of hydrolytic activity. The composition of the substrates used in the cultivation of the fluid was enriched in protein, starch or cell wall. Enzyme preparations were evaluated by incubating in 30 mL of buffer 50 mM Tris-HCl (pH 6.5) at 39 degrees C during 48 hours, 100 mg of crude protein from feeds soybean meal, canola meal, sunflower meal, gluten feed, dehydrated alfalfa meal, berseem clover, oat forage and perennial ryegrass. Enzyme extracts from cultivated rumen fluid showed an average protein breakdown of 75.5%, in eight feed samples tested. This value was very close to that measured with the technique of proteases from Streptomyces griseus (74.6% CP), but significantly lower (P <= 0.05) than the one obtained by the in situ methodology (84.8% CP). The technique with extracted rumen enzymes showed higher level of proteolysis in the early hours of incubation (6 H) compared to the other techniques. These results suggest that the enzyme preparations of ruminal origin have the ability to predict degradability of feed proteins in the rumen, particularly in the first phase when most of proteins are hydrolyzed and become available for microbial utilization.
dc.description.funderFONDECYT
dc.fuente.origenWOS
dc.identifier.issn0718-1620
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/95525
dc.identifier.wosidWOS:000286091000005
dc.issue.numero3
dc.language.isoen
dc.pagina.final70
dc.pagina.inicio57
dc.revistaCiencia e investigacion agraria
dc.rightsacceso restringido
dc.subjectIn vitro rumen proteolysis
dc.subjectruminal enzymes
dc.subjectStreptomyces griseus
dc.subjectin situ method
dc.subject.ods13 Climate Action
dc.subject.odspa13 Acción por el clima
dc.title<i>In vitro</i> protein breakdown by enzyme extracts of rumen origin: comparison with methods <i>in situ</i> and proteases of <i>Streptomyces griseus</i>
dc.typeartículo
dc.volumen37
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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