Structure-function relationship between the tobamovirus TMV-Cg coat protein and the HR-like response

Simple item page
dc.contributor.authorEhrenfeld, Nicole
dc.contributor.authorGonzalez, Angel
dc.contributor.authorCanon, Paola
dc.contributor.authorMedina, Consuelo
dc.contributor.authorPerez Acle, Tomas
dc.contributor.authorArce Johnson, Patricio
dc.date.accessioned2024-01-10T12:43:06Z
dc.date.available2024-01-10T12:43:06Z
dc.date.issued2008
dc.description.abstractThe tobamovirus TMV-Cg induces an HR-like response in Nicotiana tabacum cv. Xanthi nn sensitive plants lacking the N or N' resistance genes. This response has been characterized by the appearance of necrotic lesions in the inoculated leaf and viral systemic spread, although the defence pathways are activated in the plant. A previous study demonstrated that the coat protein (CP) of TMV-Cg (CPCg) was the elicitor of this HR-like response. We examined the influence of four specific amino acid substitutions on the structure of CPCg, as well as on the development of the host response. To gain insights into the structural implications of these substitutions, a set of molecular dynamic experiments was performed using comparative models of wild-type and mutant CPCg as well as the CP of the U1 strain of TMV (CPU1), which is not recognized by the plants. A P21L mutation produces severe changes in the three-dimensional structure of CPCg and is more unstable when this subunit is laterally associated in silico. This result may explain the observed incapacity of this mutant to assemble virions. Two other CPCg mutations (R46G and S54K) overcome recognition by the plant and do not induce an HR-like response. A double CPCg mutant P21L-S54K recovered its capacity to form virions and to induce an HR-like response. Our results suggest that the structural integrity of the CP proteins is important for triggering the HIR-like response.
dc.fechaingreso.objetodigital2024-05-23
dc.format.extent9 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1099/vir.0.83355-0
dc.identifier.eissn1465-2099
dc.identifier.issn0022-1317
dc.identifier.pubmedidMEDLINE:18272773
dc.identifier.urihttps://doi.org/10.1099/vir.0.83355-0
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/77566
dc.identifier.wosidWOS:000253998700023
dc.information.autorucCiencias Biológicas;Arce-Johnson P;S/I;54718
dc.information.autorucCiencias Biológicas;Canon P;S/I;981
dc.information.autorucCiencias Biológicas;Ehrenfeld N;S/I;18420
dc.information.autorucCiencias Biológicas;González A;S/I;1002683
dc.information.autorucCiencias Biológicas;Medina C;S/I;79724
dc.information.autorucCiencias Biológicas;Pérez-Acle T;S/I;90704
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final817
dc.pagina.inicio809
dc.publisherMICROBIOLOGY SOC
dc.revistaJOURNAL OF GENERAL VIROLOGY
dc.rightsacceso restringido
dc.subjectTOBACCO-MOSAIC-VIRUS
dc.subjectMEDIATED RESISTANCE
dc.subjectNICOTIANA-TABACUM
dc.subjectELICITOR
dc.subjectHYPERSENSITIVITY
dc.subjectIDENTIFICATION
dc.subjectRESOLUTION
dc.subjectMOVEMENT
dc.subjectHOMOLOG
dc.subjectMUTANT
dc.subject.ods13 Climate Action
dc.subject.ods02 Zero Hunger
dc.subject.odspa13 Acción por el clima
dc.subject.odspa02 Hambre cero
dc.titleStructure-function relationship between the tobamovirus TMV-Cg coat protein and the HR-like response
dc.typeartículo
dc.volumen89
sipa.codpersvinculados54718
sipa.codpersvinculados981
sipa.codpersvinculados18420
sipa.codpersvinculados1002683
sipa.codpersvinculados79724
sipa.codpersvinculados90704
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Structure-function relationship between the tobamovirus TMV-Cg coat protein and the HR-like response.pdf
Size:
3.09 KB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Artículos de revistas