Characterization of new microsatellite markers derived from sequence databases for the emu (Dromaius novaehollandiae)
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Date
2008
Journal Title
Journal ISSN
Volume Title
Publisher
WILEY
Abstract
The emu (Dromaius novaehollandiae), a member of ratite family, is native to Australia and has been introduced to other countries worldwide. In this work, 10 polymorphic microsatellite loci were isolated and characterized for emu from public sequences. Polymorphism was surveyed in 22 individuals from two different populations kept in captivity. Between two and 11 alleles were found per locus, and the observed heterozygosity ranged from 0.05 to 0.85, in accordance with expectations. These markers will be useful as tools for detecting levels of genetic variation, reconstructing pedigrees (for quantitative genetic analysis) and identifying markers associated to fitness traits in emu populations.
Description
Keywords
database search, Dromaius novaehollandiae, emu, microsatellite, ratite, SSRs, AMPLIFICATION, DNA