Overexpression of hepatic 5 alpha-reductase and 11 beta-hydroxysteroid dehydrogenase type 1 in visceral adipose tissue is associated with hyperinsulinemia in morbidly obese patients

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dc.catalogadorjwg
dc.contributor.authorBaudrand Biggs Rene Felipe
dc.contributor.authorDomínguez Ruiz-tagle, José Miguel
dc.contributor.authorCarvajal, Cristian A.
dc.contributor.authorRiquelme, Arnoldo
dc.contributor.authorCampino, Carmen
dc.contributor.authorMacchiavello, Stefano
dc.contributor.authorBozinovic, Milan
dc.contributor.authorMorales, Mauricio
dc.contributor.authorPizarro, Margarita
dc.contributor.authorSolis, Nancy
dc.contributor.authorEscalona, Alex
dc.contributor.authorBoza, Camilo
dc.contributor.authorArrese, Marco
dc.contributor.authorFardella, Carlos E.
dc.date.accessioned2024-05-30T15:55:15Z
dc.date.available2024-05-30T15:55:15Z
dc.date.issued2011
dc.description.abstract11 beta-Hydroxysteroid dehydrogenase type 1 (11 beta-HSD1) converts cortisone to cortisol, mainly in the liver and visceral adipose tissue (VAT), and has been implicated in several metabolic disorders. The absence of systemic hypercortisolism in central obesity could be due to increased inactivation of cortisol to its tetrahydrometabolites by the hepatic enzymes 5 alpha-and 5 beta-reductases. Our aim was to assess the expression of the reductases in the liver and of 11 beta-HSD1 in the liver and VAT in morbidly obese patients and to analyze their association with clinical, anthropometric, and biochemical parameters. Hepatic and VAT samples were obtained during bariatric surgery. 5 alpha- and 5 beta-reductases, 11 beta-HSD1, and 18S expression was measured using real-time polymerase chain reaction. Anthropometric and biochemical variables were analyzed. Forty-one patients were recruited (age, 41.8 +/- 10.6 years; body mass index, 42.1 +/- 6.6 kg/m(2); 71% women). The expression of hepatic 5 alpha- and 5 beta-reductases was positively correlated (r = +0.53, P = .004), and their expression levels were correlated with hepatic 11 beta-HSD1 expression (r = +0.61, P < .001 for 5 alpha-reductase and r = +0.50, P < .001 for 5 beta-reductase). Hepatic 5 alpha-reductase was associated with insulin (r = +0.34, P = .015). Visceral adipose tissue 11 beta-HSD1 expression was associated with glucose (r = +0.37, P = .025) and insulin (r = +0.54, P = .002). Our results showed that 5 alpha-reductase and VAT 11 beta-HSD1 expressions were associated with insulinemia. These findings suggest that overexpression of 5 alpha-reductase, through a higher inactivation of cortisol in the liver, could have a protective role in preserving hepatic sensitivity to insulin. The overexpression of liver reductases in obesity could be an adaptive response to an increase in cortisol production by the liver and visceral 11 beta-HSD1 to avoid systemic hypercortisolism. (C) 2011 Elsevier Inc. All rights reserved.
dc.description.funderChilean
dc.description.funderMillenium Nucleus in Immunology and Immunotherapy
dc.format.extent6 páginas
dc.fuente.origenWOS
dc.fuente.origenORCID
dc.identifier.doi10.1016/j.metabol.2011.05.001
dc.identifier.issn0026-0495
dc.identifier.pubmedidMEDLINE:21704348
dc.identifier.urihttps://doi.org/10.1016/j.metabol.2011.05.001
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/85999
dc.identifier.wosidWOS:000297528400018
dc.information.autorucEscuela de Medicina; Baudrand Biggs Rene Felipe; 0000-0002-8655-4957; 1024
dc.information.autorucEscuela de Medicina; Dominguez Ruiz-tagle Jose Miguel; 0000-0002-6157-8055; 9629
dc.issue.numero12
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final1780
dc.pagina.inicio1775
dc.publisherW B SAUNDERS CO-ELSEVIER INC
dc.revistaMETABOLISM-CLINICAL AND EXPERIMENTAL
dc.rightsacceso restringido
dc.subjectSplanchic Cortisol Production
dc.subjectMetabolic-Syndrome
dc.subjectInsulin-Resistance
dc.subjectNonalcoholic Steatohepatitis
dc.subjectDiabetes-Mellitus
dc.subjectExpression
dc.subjectDisease
dc.subjectGlucocorticoids
dc.subjectHumans
dc.subjectLiver
dc.titleOverexpression of hepatic 5 alpha-reductase and 11 beta-hydroxysteroid dehydrogenase type 1 in visceral adipose tissue is associated with hyperinsulinemia in morbidly obese patients
dc.typeartículo
dc.volumen60
sipa.codpersvinculados1024
sipa.codpersvinculados9629
sipa.trazabilidadWOS;05-06-2021
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