The Reprimo gene family member, reprimo-like (rprml), is required for blood development in embryonic zebrafish

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dc.article.number7131
dc.catalogadoraba
dc.contributor.authorStanic, Karen
dc.contributor.authorReig, German
dc.contributor.authorFigueroa, Ricardo J.
dc.contributor.authorRetamal, PedroA.
dc.contributor.authorWichmann Pérez, Ignacio Alberto
dc.contributor.authorOpazo, JuanC.
dc.contributor.authorOwen, Gareth Ivor
dc.contributor.author Corvalán R., Alejandro
dc.contributor.authorConcha, Miguel L.
dc.contributor.authorAmigo, Julio
dc.date.accessioned2024-06-10T21:25:28Z
dc.date.available2024-06-10T21:25:28Z
dc.date.issued2019
dc.description.abstractThe Reprimo gene family comprises a group of sing le-exon genes for which their physiological function remains poorly understood. Heretofore, mammalian Reprimo (RPRM) has been described as a putative p53-dependent tumor suppressor gene that functions at the G2/M cell cycle checkpoint. Another family member, Reprimo-like (RPRML), has not yet an established role in physiology or pathology. Importantly, RPRML expression pattern is conserved between zebrafish and human species. Here, using CRISPR-Cas9 and antisense morpholino oligonucleotides, we disrupt the expression of rprml in zebrafish and demonstrate that its loss leads to impaired definitive hematopoiesis. The formation of hemangioblasts and the primitive wave of hematopoiesis occur normally in absence of rprml Later in development there is a significant reduction in erythroid-myeloid precursors (EMP) at the posterior blood island (PBI) and a significant decline of definitive hematopoietic stem/progenitor cells (HSPCs). Furthermore, loss of rprml also increases the activity of caspase-3 in endothelial cells within the caudal hematopoietic tissue (CHT), the first perivascular niche where HSPCs reside during zebrafish embryonic development. Herein, we report an essential role for rprml during hematovascular development in zebrafish embryos, specifically during the definitive waves of hematopoiesis, indicating for the first time a physiological role for the rprml gene.
dc.format.extent11 páginas
dc.fuente.origenConveris
dc.fuente.origenORCID
dc.identifier.doi10.1038/s41598-019-43436-8
dc.identifier.issn2045-2322
dc.identifier.pubmedidMEDLINE:31073223
dc.identifier.urihttps://doi.org/10.1038/s41598-019-43436-8
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/86685
dc.identifier.wosidWOS:000467538500011
dc.information.autorucFACULTAD DE CIENCIAS BIOLÓGICAS;OWEN GARETH IVOR;S/I;1000459
dc.information.autorucESCUELA DE MEDICINA;CORVALAN RODRIGUEZ ALEJANDRO;S/I;63885
dc.information.autorucFACULTAD DE CIENCIAS BIOLÓGICAS;AMIGO DONOSO JULIO CRISTIAN;S/I;231252
dc.information.autorucEscuela de Medicina; Wichmann Pérez, Ignacio Alberto; 0000-0002-1115-2937; 149908
dc.information.autorucFacultad de Ciencias Biológicas; Owen, Gareth Ivor; 0000-0003-3807-6054; 1000459
dc.information.autorucFacultad de Ciencias Biológicas; Amigo, Julio; 0000-0002-6481-8034; 231252
dc.language.isoen
dc.nota.accesocontenido completo
dc.pagina.final11
dc.pagina.inicio1
dc.revistaScientific Reports
dc.rightsacceso abierto
dc.rights.licenseATTRIBUTION 4.0 INTERNATIONAL
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.en
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.titleThe Reprimo gene family member, reprimo-like (rprml), is required for blood development in embryonic zebrafish
dc.typeartículo
dc.volumen9
sipa.codpersvinculados149908
sipa.codpersvinculados1000459
sipa.codpersvinculados63885
sipa.codpersvinculados231252
sipa.trazabilidadORCID;2024-06-03
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