Macrophage migration inhibitory factor - a therapeutic target in gallbladder cancer

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dc.contributor.authorSubbannayya, Tejaswini.
dc.contributor.authorGarcía Cañete, Patricia
dc.contributor.authorRoa Strauch, Juan Carlos Enrique
dc.contributor.authorLeal Rojas, Pamela.
dc.contributor.authorBarbhuiya, Mustafa A.
dc.contributor.authorRaja, Remya.
dc.contributor.authorRenuse, Santosh.
dc.contributor.authorSathe, Gajanan.
dc.contributor.authorPinto, Sneha M.
dc.contributor.authorSyed, Nazia.
dc.date.accessioned2019-10-17T14:38:12Z
dc.date.available2019-10-17T14:38:12Z
dc.date.issued2015
dc.date.updated2019-10-14T18:36:19Z
dc.description.abstractAbstract Background Poor prognosis in gallbladder cancer is due to late presentation of the disease, lack of reliable biomarkers for early diagnosis and limited targeted therapies. Early diagnostic markers and novel therapeutic targets can significantly improve clinical management of gallbladder cancer. Methods Proteomic analysis of four gallbladder cancer cell lines based on the invasive property (non-invasive to highly invasive) was carried out using the isobaric tags for relative and absolute quantitation labeling-based quantitative proteomic approach. The expression of macrophage migration inhibitory factor was analysed in gallbladder adenocarcinoma tissues using immunohistochemistry. In vitro cellular assays were carried out in a panel of gallbladder cancer cell lines using MIF inhibitors, ISO-1 and 4-IPP or its specific siRNA. Results The quantitative proteomic experiment led to the identification of 3,653 proteins, among which 654 were found to be overexpressed and 387 were downregulated in the invasive cell lines (OCUG-1, NOZ and GB-d1) compared to the non-invasive cell line, TGBC24TKB. Among these, macrophage migration inhibitory factor (MIF) was observed to be highly overexpressed in two of the invasive cell lines. MIF is a pleiotropic proinflammatory cytokine that plays a causative role in multiple diseases, including cancer. MIF has been reported to play a central role in tumor cell proliferation and invasion in several cancers. Immunohistochemical labeling of tumor tissue microarrays for MIF expression revealed that it was overexpressed in 21 of 29 gallbladder adenocarcinoma cases. Silencing/inhibition of MIF using siRNA and/or MIF antagonists resulted in a significant decrease in cell viability, colony forming ability and invasive property of the gallbladder cancer cells. Conclusions Our findings support the role of MIF in tumor aggressiveness and suggest its potential application as a therapeutic target for gallbladder cancer.
dc.fuente.origenBiomed Central
dc.identifier.citationBMC Cancer. 2015 Nov 04;15(1):843
dc.identifier.doi10.1186/s12885-015-1855-z
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/26717
dc.identifier.urihttps://doi.org/10.1186/s12885-015-1855-z
dc.identifier.wosidWOS:000364116700004
dc.issue.numeroNo. 843
dc.language.isoen
dc.pagina.final12
dc.pagina.inicio1
dc.revistaBMC Canceres_ES
dc.rightsacceso abierto
dc.rights.holderSubbannayya et al.
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.subject.otherCáncer de vesícula biliar - Diagnósticoes_ES
dc.subject.otherCáncer de vesícula biliar- Terapiaes_ES
dc.subject.otherMacrófagoses_ES
dc.titleMacrophage migration inhibitory factor - a therapeutic target in gallbladder canceres_ES
dc.typeartículo
dc.volumenVol. 15
sipa.codpersvinculados73909
sipa.codpersvinculados84743
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