The Elav-like protein HuR exerts translational control of viral internal ribosome entry sites

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dc.contributor.authorRivas Aravena, Andrea
dc.contributor.authorRamdohr, Pablo
dc.contributor.authorVallejos, Maricarmen
dc.contributor.authorValiente Echeverria, Fernando
dc.contributor.authorDormoy Raclet, Virginie
dc.contributor.authorRodriguez, Felipe
dc.contributor.authorPino, Karla
dc.contributor.authorHolzmann, Cristian
dc.contributor.authorHuidobro Toro, J. Pablo
dc.contributor.authorGallouzi, Imed Eddine
dc.contributor.authorLopez Lastra, Marcelo
dc.date.accessioned2024-01-10T12:43:44Z
dc.date.available2024-01-10T12:43:44Z
dc.date.issued2009
dc.description.abstractThe human embryonic-lethal abnormal vision (ELAV)-like protein. HuR, has been recently found to be involved in the regulation of protein synthesis. In this study we show that HuR participates in the translational control of the HIV-1 and HCV IRES elements. HuR functions as a repressor of HIV-1]RES activity and acts as an activator of the HCV]RES. The effect of HuR was evaluated in three independent experimental systems, rabbit reticulocyte lysate, HeLa cells, and Xenopus laevis oocytes, using both overexpression and knockdown approaches. Furthermore, results suggest that HuR mediated regulation of HIV-1 and HCV IRESes does not require direct binding of the protein to the RNA nor does it need the nuclear translocation of the IRES-containing RNAs. Finally, we show that HuR has a negative impact on post-integration steps of the HIV-1 replication cycle. Thus, our observations yield novel insights into the role of HuR in the post-transcriptional regulation of HCV and HIV-1 gene expression. (C) 2009 Elsevier Inc. All rights reserved.
dc.description.funderFONDECYT
dc.description.funderFONDAP
dc.description.funderNCIC (National Cancer Institute of Canada)
dc.description.funderVRAID Pontificia Universidad Catolica de Chile
dc.description.funderMECESUP-Universidad Andres Bello
dc.description.funderMIFAB Institute
dc.description.funderCONICYT
dc.description.funderTerry Fox Foundation
dc.description.funderNational Cancer Institute of Canada
dc.fechaingreso.objetodigital17-04-2024
dc.format.extent8 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.virol.2009.06.050
dc.identifier.issn0042-6822
dc.identifier.pubmedidMEDLINE:19647848
dc.identifier.urihttps://doi.org/10.1016/j.virol.2009.06.050
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/77618
dc.identifier.wosidWOS:000270247300004
dc.information.autorucCiencias Biológicas;Holzmann C;S/I;120925
dc.information.autorucCiencias Biológicas;Huidobro-Toro JP;S/I;98862
dc.information.autorucMedicina;López-Lastra M;S/I;84823
dc.information.autorucMedicina;Pino K;S/I;1005654
dc.information.autorucCiencias Biológicas;Ramdohr P;S/I;119722
dc.information.autorucCiencias Biológicas;Rivas-Aravena A;S/I;144307
dc.information.autorucMedicina;Valiente-Echeverría F;S/I;1006402
dc.information.autorucMedicina;Vallejos M;S/I;132558
dc.issue.numero2
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.final185
dc.pagina.inicio178
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCE
dc.revistaVIROLOGY
dc.rightsacceso restringido
dc.subjectHuR
dc.subjectITAF
dc.subjectIRES
dc.subjectHIV-1
dc.subjectHCV
dc.subjectTranslation initiation
dc.subjectC VIRUS-RNA
dc.subjectCAP-INDEPENDENT TRANSLATION
dc.subjectTRANS-ACTING FACTORS
dc.subjectMESSENGER-RNA
dc.subjectMEDIATED TRANSLATION
dc.subjectBINDING-PROTEIN
dc.subjectIN-VIVO
dc.subjectINITIATION
dc.subjectMECHANISMS
dc.subjectEUKARYOTES
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleThe Elav-like protein HuR exerts translational control of viral internal ribosome entry sites
dc.typeartículo
dc.volumen392
sipa.codpersvinculados120925
sipa.codpersvinculados98862
sipa.codpersvinculados84823
sipa.codpersvinculados1005654
sipa.codpersvinculados119722
sipa.codpersvinculados144307
sipa.codpersvinculados1006402
sipa.codpersvinculados132558
sipa.indexWOS
sipa.trazabilidadCarga SIPA;09-01-2024
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