Effects of diosgenin, a plant-derived steroid, on bile secretion and hepatocellular cholestasis induced by estrogens in the rat

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dc.contributor.authorAccatino, L
dc.contributor.authorPizarro, M
dc.contributor.authorSolis, N
dc.contributor.authorKoenig, CS
dc.date.accessioned2024-01-10T12:38:35Z
dc.date.available2024-01-10T12:38:35Z
dc.date.issued1998
dc.description.abstractIncreased biliary secretion of cholesterol and lipid vesicles (unilamellae and multilamellae) induced by diosgenin (D), a plant-derived steroid, has cytoprotective effects in the rat liver subjected to obstructive cholestasis. In this study, our aims were to investigate the following: 1) the effects of D on the bile secretory process and on the cholestasis induced by estradiol-17 beta-(beta-D-glucuronide) (E17G) or 17 alpha-ethynylestradiol(E) administration; 2) whether the potentially protective effects of D are related to D-induced increase of biliary cholesterol and lipid lamellae; and 3) whether D has other effects capable of modifying specific bile secretory processes or preventing the cholestatic effects of estrogens. Rats were fed a standard ground chow (control group) or chow containing D for 6 days. E17G was administered i,v, to control and D-fed rats and bile flow, bile salt output, and alkaline phosphatase excretion were examined. 17 alpha-E was administered from days 4 to 6 to rats fed standard chow or chow plus D for 6 days and different functional parameters of the bile secretory process as well as the ultrastructure of hepatocytes and histochemistry of alkaline phosphatase and Mg2+-adenosine triphosphatase (ATPase:) were examined. D-treatment markedly increased cholesterol and lamellar structures in bile and attenuated the acute cholestatic effects of E17G, D-feeding prevented the decrease of taurocholate maximum secretory rate and the increase of biliary alkaline phosphatase and Ca2+,Mg2+-EctoATPase (EctoATPase) excretion, as well as the increase of cholesterol/ phospholipids ratio, alkaline phosphatase activity, and EctoATPase content in canalicular plasma membranes induced by E, D-feeding did not prevent E-induced decrease of basal bile flow, bile salt, cholesterol, and phospholipid secretory rates nor the decrease of Na+,K+-ATPase activity and Nac-taurocholate cotransporting polypeptide (Ntcp) content in isolated sinusoidal membranes. Cholestatic alterations of canalicular domain were apparent in E-treated rats. D administration was also associated with changes of ultraestructure and histochemistry of hepatocytes, E-induced alterations in ultrastructure and acinar distribution and intensity of histochemical reaction of both enzymes were partially prevented by D-feeding, We conclude that D administration, in addition to inducing a marked increase of biliary cholesterol and lipid lamellar structures output, was associated to changes in hepatocyte morphology and plasma membrane composition, enzymes activity, and histochemistry, D-feeding attenuated the acute cholestatic effects of E17G. D-induced increase of bile cholesterol and lipid lamellae content was not apparent when D-fed rats received E. Despite this fact, D administration prevented some cholestatic effects of E, probably through different metabolic effects and/or direct membrane effects, not related to increased lipid lamellae excretion.
dc.fechaingreso.objetodigital05-04-2024
dc.format.extent12 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1002/hep.510280118
dc.identifier.issn0270-9139
dc.identifier.pubmedidMEDLINE:9657105
dc.identifier.urihttps://doi.org/10.1002/hep.510280118
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/77067
dc.identifier.wosidWOS:000074467800018
dc.information.autorucMedicina;Accatino L;S/I;99016
dc.issue.numero1
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.final140
dc.pagina.inicio129
dc.publisherW B SAUNDERS CO
dc.revistaHEPATOLOGY
dc.rightsacceso restringido
dc.subjectHEPATIC CHOLESTEROL ESTERIFICATION
dc.subjectBILIARY CHOLESTEROL
dc.subjectPLASMA-MEMBRANE
dc.subjectSALT TRANSPORT
dc.subjectPHOSPHOLIPID LAMELLAE
dc.subjectALKALINE-PHOSPHATASE
dc.subjectADAPTIVE REGULATION
dc.subjectCELL-DAMAGE
dc.subjectLIVER
dc.subjectCARRIERS
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleEffects of diosgenin, a plant-derived steroid, on bile secretion and hepatocellular cholestasis induced by estrogens in the rat
dc.typeartículo
dc.volumen28
sipa.codpersvinculados99016
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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