Eicosapentaenoic acid promotes mitochondrial biogenesis and beige-like features in subcutaneous adipocytes from overweight subjects

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dc.contributor.authorLaiglesia, L. M.
dc.contributor.authorLorente Cebrian, S.
dc.contributor.authorPrieto Hontoria, P. L.
dc.contributor.authorFernandez Galilea, M.
dc.contributor.authorRibeiro, S. M. R.
dc.contributor.authorSainz, N.
dc.contributor.authorMartinez, J. A.
dc.contributor.authorMoreno Aliaga, M. J.
dc.date.accessioned2024-01-10T13:12:39Z
dc.date.available2024-01-10T13:12:39Z
dc.date.issued2016
dc.description.abstractEicosapentaenoic acid (EPA), a n-3 long-chain polyunsaturated fatty acid, has been reported to have beneficial effects in obesity-associated metabolic disorders. The objective of the present study was to determine the effects of EPA on the regulation of genes involved in lipid metabolism, and the ability of EPA to induce mitochondrial biogenesis and beiging in subcutaneous adipocytes from overweight subjects. Fully differentiated human subcutaneous adipocytes from overweight females (BM1: 28.1-29.8 kg/m(2)) were treated with EPA (100-200 mu M) for 24 h. Changes in mRNA expression levels of genes involved in lipogenesis, fatty acid oxidation and mitochondrial biogenesis were determined by qRT-PCR. Mitochondrial content was evaluated using MitoTracker (R) Green stain. The effects on peroxisome proliferator-activated receptor gamma, co-activator 1 alpha (PGC-1 alpha) and AMP-activated protein kinase (AMPK) were also characterized. EPA down-regulated lipogenic genes expression while up-regulated genes involved in fatty acid oxidation. Moreover, EPA-treated adipocytes showed increased mitochondria( content, accompanied by an up-regulation of nuclear respiratory factor-1, mitochondrial transcription factor A and cytochrome c oxidase IV mRNA expression. EPA also promoted the activation of master regulators of mitochondrial biogenesis such as sirtuin 1, PGC1-alpha and AMPK. In parallel, EPA induced the expression of genes that typify beige adipocytes such as fat determination factor PR domain containing 16, uncoupling protein 1 and cell death-inducing DFFA-like effector A, T-Box protein 1 and CD137. Our results suggest that EPA induces a remodeling of adipocyte metabolism preventing fat storage and promoting fatty acid oxidation, mitochondrial biogenesis and beige-like markers in human subcutaneous adipocytes from overweight subjects. (C) 2016 Elsevier Inc. All rights reserved.
dc.description.funderMinistry of Economy and Competitivity (MINECO) of the Government of Spain
dc.description.funderLinea Especial "Nutricion, Obesidad y Salud" University of Navarra
dc.description.funderCIBER Physiopathology of Obesity and Nutrition (CIBERobn)
dc.description.funderCarlos III Health Research Institute
dc.description.funderAsociacion de Amigos de la Universidad de Navarra
dc.fechaingreso.objetodigital27-03-2024
dc.format.extent7 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.jnutbio.2016.07.019
dc.identifier.eissn1873-4847
dc.identifier.issn0955-2863
dc.identifier.pubmedidMEDLINE:27637001
dc.identifier.urihttps://doi.org/10.1016/j.jnutbio.2016.07.019
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/78214
dc.identifier.wosidWOS:000386645100009
dc.information.autorucMedicina;Fernandez-Galilea M;S/I;242757
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final82
dc.pagina.inicio76
dc.publisherELSEVIER SCIENCE INC
dc.revistaJOURNAL OF NUTRITIONAL BIOCHEMISTRY
dc.rightsacceso restringido
dc.subjectN-3 PUFAs
dc.subjectEPA
dc.subjectLipogenesis
dc.subjectFatty acid oxidation
dc.subjectMitochondrial biogenesis
dc.subjectBrowning
dc.subjectPOLYUNSATURATED FATTY-ACIDS
dc.subjectWHITE ADIPOSE-TISSUE
dc.subjectDIET-INDUCED OBESITY
dc.subjectACTIVATED PROTEIN-KINASE
dc.subjectLIPID DROPLET FORMATION
dc.subjectGENE-EXPRESSION
dc.subjectINSULIN SENSITIVITY
dc.subject3T3-L1 ADIPOCYTES
dc.subjectBETA-OXIDATION
dc.subjectETHYL-ESTER
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleEicosapentaenoic acid promotes mitochondrial biogenesis and beige-like features in subcutaneous adipocytes from overweight subjects
dc.typeartículo
dc.volumen37
sipa.codpersvinculados242757
sipa.indexWOS
sipa.trazabilidadCarga SIPA;09-01-2024
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