BIOSENSORS' PREPARATION TO BE USED FOR ENTRAPMENT METHOD WITH COPOLYMERS POLYANILINE DERIVATIVES
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Date
2010
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Journal ISSN
Volume Title
Publisher
SOC CHILENA QUIMICA
Abstract
The entrapment method was used to prepare electrochemical biosensors. Using controlled potential method, copolymers were synthesized between aniline y sulfonated-anilines in aqueous media at pH 4.9 on Platinum disc. The copolymers were characterized by elemental analysis and spectroscopy. Glucose-oxidase was entrapped in the electropolymerization process, at 4.9 in aqueous media. The copolymers modified electrodes and copolymer/enzyme were studied as working electrodes for the oxidation of different H2O2 concentrations and indirect D-glucose quantification at pH 7, using controlled potential and the cyclic voltammetry methods (CV) correspondingly. A linear relationship between the hydrogen peroxide (or D-glucose) concentration and the electrochemical response was obtained at pH 7. The modified-electrodes displayed a fast time of current response, limited by the cycle numbers of the cyclic voltammetry, 232 s, and enough stability to allow for the measurements to be taken after several days.
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Keywords
Copolymers, Electrochemistry, enzyme, biosensor, CONDUCTING POLYMERS, GLUCOSE BIOSENSOR, OXIDASE, POLYMERIZATION, IMMOBILIZATION, FILM