Hypothyroidism in the Adult Rat Causes Incremental Changes in Brain-Derived Neurotrophic Factor, Neuronal and Astrocyte Apoptosis, Gliosis, and Deterioration of Postsynaptic Density

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dc.contributor.authorCortes, Claudia
dc.contributor.authorEugenin, Eliseo
dc.contributor.authorAliaga, Esteban
dc.contributor.authorCarreno, Leandro J.
dc.contributor.authorBueno, Susan M.
dc.contributor.authorGonzalez, Pablo A.
dc.contributor.authorGayol, Silvina
dc.contributor.authorNaranjo, David
dc.contributor.authorNoches, Veronica
dc.contributor.authorMarassi, Michelle P.
dc.contributor.authorRosenthal, Doris
dc.contributor.authorJadue, Cindy
dc.contributor.authorIbarra, Paula
dc.contributor.authorKeitel, Cecilia
dc.contributor.authorWohllk, Nelson
dc.contributor.authorCourt, Felipe
dc.contributor.authorKalergis, Alexis M.
dc.contributor.authorRiedel, Claudia A.
dc.date.accessioned2024-01-10T13:13:34Z
dc.date.available2024-01-10T13:13:34Z
dc.date.issued2012
dc.description.abstractBackground: Adult hypothyroidism is a highly prevalent condition that impairs processes, such as learning and memory. Even though tetra-iodothyronine (T-4) treatment can overcome the hypothyroidism in the majority of cases, it cannot fully recover the patient's learning capacity and memory. In this work, we analyzed the cellular and molecular changes in the adult brain occurring with the development of experimental hypothyroidism.
dc.description.abstractMethods: Adult male Sprague-Dawley rats were treated with 6-propyl-2-thiouracil (PTU) for 20 days to induce hypothyroidism. Neuronal and astrocyte apoptosis were analyzed in the hippocampus of control and hypothyroid adult rats by confocal microscopy. The content of brain-derived neurotrophic factor (BDNF) was analyzed using enzyme-linked immunosorbent assay (ELISA) and in situ hybridization. The glutamatergic synapse and the postsynaptic density (PSD) were analyzed by electron microscopy. The content of PSD proteins like tyrosine receptor kinase B (TrkB), p75, and N-methyl-D-aspartate receptor (NMDAr) were analyzed by immunoblot.
dc.description.abstractResults: We observed that the hippocampus of hypothyroid adult rats displayed increased apoptosis levels in neurons and astrocyte and reactive gliosis compared with controls. Moreover, we found that the amount of BDNF mRNA was higher in the hippocampus of hypothyroid rats and the content of TrkB, the receptor for BDNF, was reduced at the PSD of the CA3 region of hypothyroid rats, compared with controls. We also observed that the glutamatergic synapses from the stratum radiatum of CA3 from hypothyroid rats, contained thinner PSDs than control rats. This observation was in agreement with a reduced content of NMDAr subunits at the PSD in hypothyroid animals.
dc.description.abstractConclusions: Our data suggest that adult hypothyroidism affects the hippocampus by a mechanism that alters the composition of PSD, reduces neuronal and astrocyte survival, and alters the content of the signaling neurotrophic factors, such as BDNF.
dc.description.funderFONDECYT
dc.description.funderMillennium Institute on Immunology and Immunotherapy
dc.description.funderMillennium Nucleus
dc.description.funderProyecto Interno Universidad Andres Bello
dc.description.funderNATIONAL INSTITUTE OF MENTAL HEALTH
dc.fechaingreso.objetodigital2024-04-26
dc.format.extent13 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1089/thy.2010.0400
dc.identifier.eissn1557-9077
dc.identifier.issn1050-7256
dc.identifier.pubmedidMEDLINE:22870949
dc.identifier.urihttps://doi.org/10.1089/thy.2010.0400
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/78317
dc.identifier.wosidWOS:000308371700014
dc.information.autorucCiencias Biológicas;Bueno S;S/I;113541
dc.information.autorucCiencias Biológicas;Carreno L;S/I;120819
dc.information.autorucCiencias Biológicas;Court F;S/I;8390
dc.information.autorucCiencias Biológicas;Gonzalez P;S/I;15616
dc.information.autorucCiencias Biológicas;Kalergis A;S/I;90610
dc.issue.numero9
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final963
dc.pagina.inicio951
dc.publisherMARY ANN LIEBERT, INC
dc.revistaTHYROID
dc.rightsacceso restringido
dc.subjectMESSENGER-RNA EXPRESSION
dc.subjectTHYROID-HORMONE
dc.subjectINDUCED IMPAIRMENT
dc.subjectDENTATE GYRUS
dc.subjectRECEPTORS
dc.subjectACTIVATION
dc.subjectP75(NTR)
dc.subjectNEUROGENESIS
dc.subjectHIPPOCAMPUS
dc.subjectMODULATION
dc.subject.ods05 Gender Equality
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa05 Igualdad de género
dc.subject.odspa03 Salud y bienestar
dc.titleHypothyroidism in the Adult Rat Causes Incremental Changes in Brain-Derived Neurotrophic Factor, Neuronal and Astrocyte Apoptosis, Gliosis, and Deterioration of Postsynaptic Density
dc.typeartículo
dc.volumen22
sipa.codpersvinculados113541
sipa.codpersvinculados120819
sipa.codpersvinculados8390
sipa.codpersvinculados15616
sipa.codpersvinculados90610
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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