A vaccine against the salmonid pathogen Piscirickettsia salmonis based on recombinant proteins

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dc.contributor.authorWilhelm, Vivian
dc.contributor.authorMiquel, Alvaro
dc.contributor.authorBurzio, Luis O.
dc.contributor.authorRosemblatt, Mario
dc.contributor.authorEngel, Esteban
dc.contributor.authorValenzuela, Sebastián
dc.contributor.authorParada, Gustavo
dc.contributor.authorValenzuela, Pablo D. T.
dc.date.accessioned2024-01-10T12:04:25Z
dc.date.available2024-01-10T12:04:25Z
dc.date.issued2006
dc.description.abstractWe report here the protective effect against piscirickettsiosis elicited in fish by a mixture of recombinant proteins. A comparative genomics strategy was used on a genomic library of Piscirickettsia salmonis in order to select optimal candidates for a recombinant subunit vaccine to protect fish from rickettsial septicaemia (SRS). Based on this information, 15 P. salmonis ORFs encoding heat shock proteins, virulence factors, membrane bound and other surface exposed antigens, were isolated and expressed. Seven of the most promising antigens were formulated in three mixtures (V1-V3) containing two or three recombinant proteins each and injected into salmon to test their protective efficacy. Two of the three formulations (V1, V2) elicited a strong protective response in a challenge against the pathogen, which was coincident with the humoral response against the corresponding recombinant proteins present in each formulation. V I, formulated with recombinant chaperonines Hsp60, Hsp70 and flagellar protein FlgG of P. salmonis achieved the highest level of protection with a relative percent survival (RPS) of 95%. (c) 2006 Elsevier Ltd. All rights reserved.
dc.fechaingreso.objetodigital11-04-2024
dc.format.extent9 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.vaccine.2006.03.027
dc.identifier.eissn1873-2518
dc.identifier.issn0264-410X
dc.identifier.pubmedidMEDLINE:16697089
dc.identifier.urihttps://doi.org/10.1016/j.vaccine.2006.03.027
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/75792
dc.identifier.wosidWOS:000238557500023
dc.information.autorucFacultad de comunicaciones ; Valenzuela, Sebastián ; 0000-0001-5991-7364 ; 6859
dc.issue.numero23
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.final5091
dc.pagina.inicio5083
dc.publisherELSEVIER SCI LTD
dc.revistaVACCINE
dc.rightsacceso restringido
dc.subjectPiscirickettsia salmonis
dc.subjectrecombinant proteins
dc.subjectsubunit vaccine
dc.subjectrecombinant antigens
dc.subjectLEGIONELLA-PNEUMOPHILA
dc.subjectMONOCLONAL-ANTIBODIES
dc.subjectNUCLEOTIDE-SEQUENCE
dc.subjectESCHERICHIA-COLI
dc.subjectMEMBRANE-PROTEIN
dc.subjectATLANTIC SALMON
dc.subjectFISH
dc.subjectGENE
dc.subjectEXPRESSION
dc.subjectVIRULENCE
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleA vaccine against the salmonid pathogen Piscirickettsia salmonis based on recombinant proteins
dc.typeartículo
dc.volumen24
sipa.codpersvinculados6859
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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