The carbon source influences the efflux pump-mediated antimicrobial resistance in clinically important Gram-negative bacteria

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dc.contributor.authorVillagra, Nicolas A.
dc.contributor.authorFuentes, Juan A.
dc.contributor.authorJofre, Matias R.
dc.contributor.authorHidalgo, Alejandro A.
dc.contributor.authorGarcia, Patricia
dc.contributor.authorMora, Guido C.
dc.date.accessioned2024-01-10T12:43:32Z
dc.date.available2024-01-10T12:43:32Z
dc.date.issued2012
dc.description.abstractMultidrug efflux pumps are proteins known to play an important role in resistance in bacteria. These proteins are located in the inner membrane (IM), together with many other proteins, including inducible permeases that participate in the uptake of non-phosphotransferase system (PTS) carbohydrates (i.e. carbohydrates uptaken by mechanisms other than the PTS). However, lipid bilayer space in the IM is limited. Therefore, we examined whether the overexpression of unrelated IM proteins is able to interfere with the efflux-mediated resistance mechanism, consequently increasing the susceptibility towards different antimicrobial compounds.
dc.description.abstractWe cultured bacteria under different conditions that increase the synthesis of unrelated IM proteins, either by using a non-PTS carbohydrate as the sole carbon source or by artificially overexpressing IM proteins, prior to determining the resistance to different antimicrobial compounds by disc diffusion assays.
dc.description.abstractWe observed that efflux-pump-mediated resistance is affected by the carbon source in all the strains tested, exhibiting increased susceptibility when a non-PTS carbohydrate was used as the sole carbon source. Moreover, when we artificially overexpressed an unrelated IM protein, we also observed decreased efflux-mediated resistance.
dc.description.abstractThese results strongly suggest that overexpression of IM proteins, by using a non-PTS carbohydrate as the sole carbon source, or by artificially introducing a high number of copies of an unrelated IM protein, competes with the antibiotic efflux systems, thereby decreasing the efflux-mediated resistance to different antimicrobial compounds. This sort of competition arises because of the limited available space in the bacterial IM, or by an unknown mechanism.
dc.description.funderFONDECYT
dc.description.funderUNAB
dc.fechaingreso.objetodigital2024-05-02
dc.format.extent7 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1093/jac/dkr573
dc.identifier.eissn1460-2091
dc.identifier.issn0305-7453
dc.identifier.pubmedidMEDLINE:22258924
dc.identifier.urihttps://doi.org/10.1093/jac/dkr573
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/77602
dc.identifier.wosidWOS:000301684900019
dc.information.autorucMedicina;Garcia P ;S/I;73909
dc.issue.numero4
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final927
dc.pagina.inicio921
dc.publisherOXFORD UNIV PRESS
dc.revistaJOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
dc.rightsacceso restringido
dc.subjectalternative carbon source
dc.subjectpermeases
dc.subjectantibiotics
dc.subjectresistance mechanisms
dc.subjectESCHERICHIA-COLI K-12
dc.subjectPSEUDOMONAS-AERUGINOSA
dc.subjectMULTIDRUG-RESISTANCE
dc.subjectMOLECULAR-MECHANISMS
dc.subjectMEMBRANE-PROTEINS
dc.subjectGENOME SEQUENCE
dc.subjectT-POP
dc.subjectXYLOSE
dc.subjectGENES
dc.subjectEXPRESSION
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleThe carbon source influences the efflux pump-mediated antimicrobial resistance in clinically important Gram-negative bacteria
dc.typeartículo
dc.volumen67
sipa.codpersvinculados73909
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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