Búsqueda de mutaciones en el gen UL 97 asociadas a resistencia a ganciclovir en citomegalovirus obtenidos desde muestras biológicas de pacientes chilenos

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dc.catalogadoraba
dc.contributor.authorOyarzún Andrade, María Angélica
dc.contributor.authorBustos, Patricia
dc.contributor.authorGonzález Agüero, Marcela Margot
dc.contributor.authorDomínguez Moreno, María Isabel
dc.contributor.authorAguayo González, Francisco Renan
dc.contributor.authorNervi Nattero, Bruno
dc.contributor.authorFerrés, Marcela
dc.date.accessioned2023-10-02T15:34:23Z
dc.date.available2023-10-02T15:34:23Z
dc.date.issued2010
dc.description.abstractBackground: Long term use of ganciclovir (GCV) is associated with acquired resistance to it. Ninety percent of the responsible mutations occur in cytomegalovirus (CMV) UL97 gene. Aim: To search for these mutations, comparing nucleotide sequences of CMV-positive samples from post transplant and immunocompromised patients receiving GCV, with sequences of CMV isolates obtained from subjects not exposed to the drug. Patients and Methods: Codons 440 to 465 of gene UL97, including the most common mutations causing resistance to GCV, were amplified in 33 plasma samples from patients exposed to GCV and in 15 urine samples of newborns. Both populations and their nucleotide sequences were compared with the prototype strain CMV AD169. Results: Samples of exposed patients had multiple mutations but only one had a mutation associated with clinical resistance (M4601). Eight subjects had the D605E mutation, whose role in resistance is controversial. The remaining 150 mutations were silent mutations. Conclusions: A low frequency of mutations associated with CMV resistance to GCV was found in these exposed and unexposed samples. These mutations may reflect coexistence of multiple genetic variants of CMV. The absence of clinical expression of resistance, even with these mutations, can be explained by the use of GCV for a shorter lapse than that associated with the appearance of resistance. (Rev Med Chile 2010; 138: 421-427).
dc.fechaingreso.objetodigital2023-10-02
dc.format.extent7 páginas
dc.fuente.origenWOS
dc.identifier.eissn0717-6163
dc.identifier.issn0034-9887
dc.identifier.pubmedidMEDLINE:20668789
dc.identifier.scopusidSCOPUS_ID:77954422871
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/74824
dc.identifier.wosidWOS:000278240800005
dc.information.autorucEscuela de Medicina; Oyarzún Andrade, María Angélica; 0009-0008-6301-000X; 2422
dc.information.autorucEscuela de Enfermería; González Agüero, Marcela Margot; 0000-0002-0738-4399; 119783
dc.information.autorucEscuela de Medicina; Domínguez Moreno, María Isabel; S/I; 12893
dc.information.autorucEscuela de Medicina; Aguayo González, Francisco Renan; 0000-0002-9619-7535; 1005475
dc.information.autorucEscuela de Medicina; Nervi Nattero, Bruno; 0000-0002-3016-7261; 604
dc.information.autorucEscuela de Medicina; Ferrés, Marcela; 0000-0001-9415-4657; 66180
dc.issue.numero4
dc.language.isoes
dc.nota.accesoContenido completo
dc.pagina.final427
dc.pagina.inicio421
dc.revistaRevista Medica de Chile
dc.rightsacceso abierto
dc.subjectAnti viral drug resistance
dc.subjectCytomegalovirus
dc.subjectGanciclovir
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleBúsqueda de mutaciones en el gen UL 97 asociadas a resistencia a ganciclovir en citomegalovirus obtenidos desde muestras biológicas de pacientes chilenos
dc.title.alternativePresence of mutations associated with gancicloyir resistance in cytomegalovirus UL97 gene
dc.typeartículo
dc.volumen138
sipa.codpersvinculados2422
sipa.codpersvinculados119783
sipa.codpersvinculados12893
sipa.codpersvinculados1005475
sipa.codpersvinculados604
sipa.codpersvinculados66180
sipa.indexWoS
sipa.indexPubmed
sipa.indexSCOPUS
sipa.indexScielo
sipa.trazabilidadWOS;05-06-2021
sipa.trazabilidadORCID;2023-10-02
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