Prevalence of heparin-induced antibodies in patients with chronic renal failure undergoing hemodialysis

Simple item page
dc.contributor.authorPalomo, I
dc.contributor.authorPereira, J
dc.contributor.authorAlarcon, M
dc.contributor.authorDiaz, G
dc.contributor.authorHidalgo, P
dc.contributor.authorPizarro, I
dc.contributor.authorJara, E
dc.contributor.authorRojas, P
dc.contributor.authorQuiroga, G
dc.contributor.authorMoore Carrasco, R
dc.date.accessioned2024-01-10T13:16:32Z
dc.date.available2024-01-10T13:16:32Z
dc.date.issued2005
dc.description.abstractHeparin-induced thrombocytopenia (HIT) type II is a serious complication of heparin therapy. It presents initially as thrombocytopenia, and is associated with thrombosis in 20-50% of the cases. HIT is related to the presence of heparin-induced antibodies (HIA), which show specificity for the PF4-heparin (PF4-H) complex. The Fc gamma RIIa receptor has been suggested to participate in the pathogenic mechanism of HIA. Since patients undergoing chronic hemodialysis (HD) are exposed repeatedly to heparin, we studied the prevalence of HIA and their eventual relationship with thrombocytopenia and/or thrombosis, and the possible participation of the Fc gamma RIIa polymorphism. We studied 207 patients with chronic renal failure (CRF) undergoing HID. As a control we included 130 blood donors and 28 patients with CRF without HID. The HIA patients were studied with the use of a PF4-H ELISA. Additionally, in some positive cases for the previous test, a C-14- serotonin release assay (C-14-SRA) was performed. The polymorphism Fc gamma RIIa H/R131 was studied by polymerase chain reaction (PCR) with allele-specific primers. Thirty-seven patients (17.9%) undergoing HD presented with HIA. The majority of these antibodies were IgG, IgM, and IgA. The HIA investigated presented specificity against the PF4-H complex, but not against PF4 alone (P < 0.001). Twelve out of 22 (54.5%) PF4-H antibodies were positive when tested with the C-14-SRA. The distribution of the Fc gamma RIIa polymorphism in patients and healthy controls was 42.6% and 41.6% for H/H131, 41% and 48.9% for the H/R131 isoform, and 16.4% and 9.5% for the R/R131 isoform, respectively. No statistically significant difference in the Fc gamma RIIa isoform distribution was found. Twenty-nine out of 156 patients (18.5%) presented thrombocytopenia, and 21/207 (12.4%) had thrombosis of the native vein arterio-venous fistula (AVF). We did not find any statistically significant between HIA and thrombocytopenia or thrombosis. An important proportion of patients with CRF undergoing HD developed HIA, but these cases were not associated with thrombocytopenia or thrombosis of AVF. The frequency of the Fc gamma RIIa polymorphism did not statistically differ between HIT type II and normal controls.
dc.fechaingreso.objetodigital2024-04-27
dc.format.extent7 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1002/jcla.20076
dc.identifier.eissn1098-2825
dc.identifier.issn0887-8013
dc.identifier.pubmedidMEDLINE:16170812
dc.identifier.urihttps://doi.org/10.1002/jcla.20076
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/78593
dc.identifier.wosidWOS:000232330900003
dc.information.autorucMedicina;Pereira J;S/I;99371
dc.issue.numero5
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.final195
dc.pagina.inicio189
dc.publisherWILEY
dc.revistaJOURNAL OF CLINICAL LABORATORY ANALYSIS
dc.rightsacceso restringido
dc.subjectheparin-induced antibodies
dc.subjectFc gamma RIIa
dc.subjecthemodialysis
dc.subjectFC-GAMMA-RECEPTOR
dc.subjectSINGLE AMINO-ACID
dc.subjectINDUCED THROMBOCYTOPENIA
dc.subjectPLATELET-AGGREGATION
dc.subjectENDOTHELIAL-CELLS
dc.subjectPOLYMORPHISM
dc.subjectIGG
dc.subjectPLATELET-FACTOR-4
dc.subjectANTIGEN
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titlePrevalence of heparin-induced antibodies in patients with chronic renal failure undergoing hemodialysis
dc.typeartículo
dc.volumen19
sipa.codpersvinculados99371
sipa.indexWOS
sipa.trazabilidadCarga SIPA;09-01-2024
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Prevalence of heparin-induced antibodies in patients with chronic renal failure undergoing hemodialysis.pdf
Size:
3.57 KB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Artículos de revistas