Chromosomal excision of a new pathogenicity island modulates Salmonella virulence in vivo

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dc.catalogadorpva
dc.contributor.authorTobar Durán, Hugo Eduardo
dc.contributor.authorSalazar Echegarai, Francisco J.
dc.contributor.authorNieto Pacheco, Pamela Andrea
dc.contributor.authorPalavecino, Christian E.
dc.contributor.authorSebastian, Vatenlina P.
dc.contributor.authorRiedel Soria, Claudia
dc.contributor.authorKalergis Parra, Alexis Mikes
dc.contributor.authorBueno Ramírez, Susan
dc.date.accessioned2024-01-24T19:26:06Z
dc.date.available2024-01-24T19:26:06Z
dc.date.issued2013
dc.description.abstractAlthough the excision of unstable pathogenicity islands is a phenomenon that has been described for several virulent bacteria, whether this process directly affects the capacity of these microorganisms to cause disease in their hosts remains unknown. Salmonella enterica serovar Enteritidis (S. Enteritidis) is an enterobacterium that harbors several unstable pathogenicity islands that can excise from the main bacterial chromosome. Here we have evaluated whether excision of one of these pathogenicity islands, denominated as Region of Difference 21 (ROD21), is required for S. Enteritidis to cause disease in the host. By means of genetic targeting of the integrase encoded by the ROD21 we have generated S. Enteritidis strains unable to excise ROD21. The failure to excise ROD21 significantly reduced the capacity to cause a lethal disease and to colonize the spleen and liver of mice, as compared to wild type S. Enteritidis. On the contrary, S. Enteritidis strains overexpressing an excisionase protein increased the frequency of ROD21 excision and showed an improved capacity to cause lethal disease in mice. Accordingly, strains unable to excise ROD21 showed an altered expression of genes located in this pathogenicity island. Our results suggest that the genetic excision of the pathogenicity island ROD21 in S. Enteritidis modulates the capacity of this bacterium to cause disease in mice due to a change in the expression of virulence genes.
dc.description.funderFONDECYT
dc.description.funderMillennium Institute on Immunology and Immunotherapy
dc.fechaingreso.objetodigital2024-01-24
dc.fuente.origenWOS
dc.identifier.doi10.2174/1566523211313040002
dc.identifier.eissn1875-5631
dc.identifier.issn1566-5232
dc.identifier.pubmedidMEDLINE:23746206
dc.identifier.urihttps://doi.org/10.2174/1566523211313040002
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/80943
dc.identifier.wosidWOS:000324167000002
dc.information.autorucFacultad de Ciencias Biológicas; Tobar Durán, Hugo Eduardo; 0000-0002-9958-6253; 181479
dc.information.autorucFacultad de Ciencias Biológicas; Nieto Pacheco, Pamela Andrea; S/I; 120888
dc.information.autorucFacultad de Ciencias Biológicas; Riedel Soria, Claudia; S/I; 88772
dc.information.autorucFacultad de Ciencias Biológicas; Kalergis Parra, Alexis Mikes; 0000-0001-7622-5263; 90610
dc.information.autorucFacultad de Ciencias Biológicas; Bueno Ramírez, Susan; 0000-0002-7551-8088; 113541
dc.issue.numero4
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.final249
dc.pagina.inicio240
dc.publisherBENTHAM SCIENCE PUBL LTD
dc.revistaCurrent Gene Therapyes_ES
dc.rightsacceso restringido
dc.subjectSalmonella enterica serovar enteritidises_ES
dc.subjectSpathogenicity island excisiones_ES
dc.subjectSystemic infectiones_ES
dc.subjectENTERICA SEROVAR ENTERITIDISes_ES
dc.subjectHOST ADAPTATIONes_ES
dc.subjectGENOMIC ISLANDes_ES
dc.subjectGENEes_ES
dc.subjectTYPHIMURIUMes_ES
dc.subjectFAMILYes_ES
dc.subjectMICEes_ES
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleChromosomal excision of a new pathogenicity island modulates Salmonella virulence in vivoes_ES
dc.typeartículo
dc.volumen13
sipa.codpersvinculados237763
sipa.codpersvinculados1011015
sipa.codpersvinculados171925
sipa.codpersvinculados171925
sipa.codpersvinculados181479
sipa.codpersvinculados120888
sipa.codpersvinculados88772
sipa.codpersvinculados90610
sipa.codpersvinculados113541
sipa.trazabilidadWOS;05-06-2021
sipa.trazabilidadORCID;2024-01-15
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