Genetic and histological studies on the delayed systemic movement of Tobacco Mosaic Virus in Arabidopsis thaliana

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dc.contributor.authorSerrano Honeyman, Carolina
dc.contributor.authorGonzález Cruz, Javiera
dc.contributor.authorJauregui, Francisca
dc.contributor.authorMedina, Consuelo
dc.contributor.authorMancilla Oyarzo, Pablo Eduardo
dc.contributor.authorMatus Picero, José Tomás
dc.contributor.authorArce Johnson, Jorge Patricio
dc.date.accessioned2019-10-17T15:26:51Z
dc.date.available2019-10-17T15:26:51Z
dc.date.issued2008
dc.date.updated2019-10-14T18:37:38Z
dc.description.abstractAbstract Background Viral infections and their spread throughout a plant require numerous interactions between the host and the virus. While new functions of viral proteins involved in these processes have been revealed, current knowledge of host factors involved in the spread of a viral infection is still insufficient. In Arabidopsis thaliana, different ecotypes present varying susceptibilities to Tobacco mosaic virus strain U1 (TMV-U1). The rate of TMV-U1 systemic movement is delayed in ecotype Col-0 when compared with other 13 ecotypes. We followed viral movement through vascular tissue in Col-0 plants by electronic microscopy studies. In addition, the delay in systemic movement of TMV-U1 was genetically studied. Results TMV-U1 reaches apical leaves only after 18 days post rosette inoculation (dpi) in Col-0, whereas it is detected at 9 dpi in the Uk-4 ecotype. Genetic crosses between Col-0 and Uk-4 ecotypes, followed by analysis of viral movement in F1 and F2 populations, revealed that this delayed movement correlates with a recessive, monogenic and nuclear locus. The use of selected polymorphic markers showed that this locus, denoted DSTM1 (Delayed Systemic Tobamovirus Movement 1), is positioned on the large arm of chromosome II. Electron microscopy studies following the virion's route in stems of Col-0 infected plants showed the presence of curved structures, instead of the typical rigid rods of TMV-U1. This was not observed in the case of TMV-U1 infection in Uk-4, where the observed virions have the typical rigid rod morphology. Conclusion The presence of defectively assembled virions observed by electron microscopy in vascular tissue of Col-0 infected plants correlates with a recessive delayed systemic movement trait of TMV-U1 in this ecotype.Abstract Background Viral infections and their spread throughout a plant require numerous interactions between the host and the virus. While new functions of viral proteins involved in these processes have been revealed, current knowledge of host factors involved in the spread of a viral infection is still insufficient. In Arabidopsis thaliana, different ecotypes present varying susceptibilities to Tobacco mosaic virus strain U1 (TMV-U1). The rate of TMV-U1 systemic movement is delayed in ecotype Col-0 when compared with other 13 ecotypes. We followed viral movement through vascular tissue in Col-0 plants by electronic microscopy studies. In addition, the delay in systemic movement of TMV-U1 was genetically studied. Results TMV-U1 reaches apical leaves only after 18 days post rosette inoculation (dpi) in Col-0, whereas it is detected at 9 dpi in the Uk-4 ecotype. Genetic crosses between Col-0 and Uk-4 ecotypes, followed by analysis of viral movement in F1 and F2 populations, revealed that this delayed movement correlates with a recessive, monogenic and nuclear locus. The use of selected polymorphic markers showed that this locus, denoted DSTM1 (Delayed Systemic Tobamovirus Movement 1), is positioned on the large arm of chromosome II. Electron microscopy studies following the virion's route in stems of Col-0 infected plants showed the presence of curved structures, instead of the typical rigid rods of TMV-U1. This was not observed in the case of TMV-U1 infection in Uk-4, where the observed virions have the typical rigid rod morphology. Conclusion The presence of defectively assembled virions observed by electron microscopy in vascular tissue of Col-0 infected plants correlates with a recessive delayed systemic movement trait of TMV-U1 in this ecotype.
dc.fuente.origenBiomed Central
dc.identifier.citationBMC Genetics. 2008 Sep 26;9(1):59
dc.identifier.doi10.1186/1471-2156-9-59
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/26772
dc.issue.numeroNo. 59
dc.language.isoen
dc.nota.accesoContenido completo
dc.pagina.final11
dc.pagina.inicio1
dc.revistaBMC Geneticses_ES
dc.rightsacceso abierto
dc.rights.holderSerrano et al; licensee BioMed Central Ltd.
dc.subject.ddc570
dc.subject.deweyBiologíaes_ES
dc.subject.otherPlantases_ES
dc.subject.otherPlantas viruses_ES
dc.subject.otherVirus del mosaico del tabacoes_ES
dc.titleGenetic and histological studies on the delayed systemic movement of Tobacco Mosaic Virus in Arabidopsis thalianaes_ES
dc.typeartículo
dc.volumenVol. 9
sipa.codpersvinculados15296
sipa.codpersvinculados54718
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