Differential expression of functional nucleoside transporters in non-differentiated and differentiated human endothelial progenitor cells

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dc.contributor.authorGuzman Gutierrez, E.
dc.contributor.authorSandoval, C.
dc.contributor.authorNova, E.
dc.contributor.authorCastillo, J. L.
dc.contributor.authorVera, J. C.
dc.contributor.authorLamperti, L.
dc.contributor.authorKrause, B.
dc.contributor.authorSalomon, C.
dc.contributor.authorSepulveda, C.
dc.contributor.authorAguayo, C.
dc.contributor.authorSobrevia, L.
dc.date.accessioned2024-01-10T13:47:35Z
dc.date.available2024-01-10T13:47:35Z
dc.date.issued2010
dc.description.abstractExtracellular adenosine removal is via human equilibrative nucleoside transporters 1 (hENT1) and 2 (hENT2) in the endothelium, thus regulating adenosine-induced revascularization and angiogenesis. Since human endothelial progenitor cells (hEPCs) promote revascularization, we hypothesize differential expression of nucleoside transporters in hEPCs. hEPCs were cultured 3 (hEPC-3d) or 14 (hEPC-14d) days. RT-PCR for prominin 1, CD34, octamer-4, kinase insert domain receptor, oxidized low-density lipoprotein (lectin-like) receptor 1 and tyrosine endothelial kinase was used to evaluate phenotypic differentiation. Flow cytometry was used to estimate CD34(+)/KDR- (non-differentiated), CD34(-)/KDR+ (differentiated) or CD34(+)/KDR+ (mixed) cell populations. Adenosine transport was measured in absence or presence of sodium, S-(4-nitrobenzyl)-6-thio-inosine (NBTI, 1-10 mu M), inosine, hypoxanthine or guanine (0.1-5 mM), hENTs protein abundance by western blot, and hENTs, hCNT1, hCNT2 and hCNT3 mRNA expression by real time RT-PCR. hEPC-3d cells were CD34(+)/KDR- compared with hEPC-14d cells that were CD34(-)/KDR+ hEPC-3d cells exhibit hENT1-like adenosine transport (NBTI-sensitive, Na+-independent), which is absent in hEPC-14d cells. hEPC-14d cells exhibit two transport components: component 1 (NBTI insensitive, Na+-independent) and component 2 (NBTI insensitive, Na+-dependent, Hill coefficient similar to 1.8), the latter resembling CNT3-like transport. hEPC-3d cells express hENT1 protein and mRNA, which is reduced (similar to 90%) in hEPC-14d cells, but instead only hCNT3 mRNA is expressed in this cell type. hENT2, hCNT1 and hCNT2 were undetectable in hEPCs. Thus, hEPCs exhibit a differential expression of hENT1 and hCNT3 functional nucleoside transporters, which could be related with its differentiation stage. (c) 2010 Elsevier Ltd. All rights reserved.
dc.description.funderFondo Nacional de Desarrollo Cientifico y Tecnologico
dc.description.funderDireccion de Investigacion Universidad de Concepcion
dc.description.funderComision Nacional de Investigacion en Ciencia y Tecnologia (CONICYT), Chile
dc.description.funderCONICYT-PhD (Chile)
dc.description.funderPostgraduate School, Universidad de Concepcion-MSc (Chile)
dc.description.funderFaculty of Medicine, Pontificia Universidad Catolica de Chile-PhD fellowship
dc.fechaingreso.objetodigital05-04-2024
dc.format.extent9 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.placenta.2010.07.016
dc.identifier.eissn1532-3102
dc.identifier.issn0143-4004
dc.identifier.pubmedidMEDLINE:20728214
dc.identifier.urihttps://doi.org/10.1016/j.placenta.2010.07.016
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/79285
dc.identifier.wosidWOS:000283408300013
dc.information.autorucMedicina;Aguayo C;S/I;118121
dc.information.autorucCiencias Biológicas;Krause B;S/I;1005678
dc.information.autorucMedicina;Salomon C;S/I;189546
dc.information.autorucMedicina;Sepulveda C;S/I;132789
dc.information.autorucMedicina;Sobrevia L;S/I;1002656
dc.issue.numero10
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.final936
dc.pagina.inicio928
dc.publisherW B SAUNDERS CO LTD
dc.revistaPLACENTA
dc.rightsacceso restringido
dc.subjectEndothelial progenitor cells
dc.subjectAdenosine
dc.subjectTransport
dc.subjectDifferentiation
dc.subjectSMOOTH-MUSCLE-CELLS
dc.subjectADENOSINE TRANSPORT
dc.subjectNITRIC-OXIDE
dc.subjectANGIOGENESIS
dc.subjectPREECLAMPSIA
dc.subjectVASCULOGENESIS
dc.subjectDYSFUNCTION
dc.subjectINHIBITORS
dc.subjectPREGNANCY
dc.subjectLEUKEMIA
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleDifferential expression of functional nucleoside transporters in non-differentiated and differentiated human endothelial progenitor cells
dc.typeartículo
dc.volumen31
sipa.codpersvinculados118121
sipa.codpersvinculados1005678
sipa.codpersvinculados189546
sipa.codpersvinculados132789
sipa.codpersvinculados1002656
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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