The vaginal immunoproteome for the prediction of spontaneous preterm birth: A retrospective longitudinal study

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dc.contributor.authorShaffer, Zachary
dc.contributor.authorRomero, Roberto
dc.contributor.authorTarca, Adi L.
dc.contributor.authorGalaz, Jose
dc.contributor.authorArenas-Hernandez, Marcia
dc.contributor.authorGudicha, Dereje W.
dc.contributor.authorChaiworapongsa, Tinnakorn
dc.contributor.authorJung, Eunjung
dc.contributor.authorSuksai, Manaphat
dc.contributor.authorTheis, Kevin R.
dc.contributor.authorGomez-Lopez, Nardhy
dc.contributor.authorSimon, Carlos
dc.date.accessioned2025-01-20T16:14:36Z
dc.date.available2025-01-20T16:14:36Z
dc.date.issued2024
dc.description.abstractBackground: Preterm birth is the leading cause of neonatal morbidity and mortality worldwide. Most cases of preterm birth occur spontaneously and result from preterm labor with intact (spontaneous preterm labor [sPTL]) or ruptured (preterm prelabor rupture of membranes [PPROM]) membranes. The prediction of spontaneous preterm birth (sPTB) remains underpowered due to its syndromic nature and the dearth of independent analyses of the vaginal host immune response. Thus, we conducted the largest longitudinal investigation targeting vaginal immune mediators, referred to herein as the immunoproteome, in a population at high risk for sPTB. Methods: Vaginal swabs were collected across gestation from pregnant women who ultimately underwent term birth, sPTL, or PPROM. Cytokines, chemokines, growth factors, and antimicrobial peptides in the samples were quantified via specific and sensitive immunoassays. Predictive models were constructed from immune mediator concentrations. Results: Throughout uncomplicated gestation, the vaginal immunoproteome harbors a cytokine network with a homeostatic profile. Yet, the vaginal immunoproteome is skewed toward a pro-inflammatory state in pregnant women who ultimately experience sPTL and PPROM. Such an inflammatory profile includes increased monocyte chemoattractants, cytokines indicative of macrophage and T-cell activation, and reduced antimicrobial proteins/peptides. The vaginal immunoproteome has improved predictive value over maternal characteristics alone for identifying women at risk for early (<34 weeks) sPTB. Conclusions: The vaginal immunoproteome undergoes homeostatic changes throughout gestation and deviations from this shift are associated with sPTB. Furthermore, the vaginal immunoproteome can be leveraged as a potential biomarker for early sPTB, a subset of sPTB associated with extremely adverse neonatal outcomes.
dc.description.funderEunice Kennedy Shriver National Institute of Child Health and Human Development
dc.fuente.origenWOS
dc.identifier.doi10.7554/eLife.90943.sa2
dc.identifier.issn2050-084X
dc.identifier.urihttps://doi.org/10.7554/eLife.90943
dc.identifier.urihttps://doi.org/10.7554/eLife.90943.sa1
dc.identifier.urihttps://doi.org/10.7554/eLife.90943.sa2
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/90450
dc.identifier.wosidWOS:001255146200001
dc.language.isoen
dc.revistaElife
dc.rightsacceso restringido
dc.subjectpreterm birth
dc.subjectimmunology
dc.subjectvaginal ecosystem
dc.subjectobstetrics
dc.subjectgreat obstetrical syndromes
dc.subjectHuman
dc.subject.ods03 Good Health and Well-being
dc.subject.ods05 Gender Equality
dc.subject.odspa03 Salud y bienestar
dc.subject.odspa05 Igualdad de género
dc.titleThe vaginal immunoproteome for the prediction of spontaneous preterm birth: A retrospective longitudinal study
dc.typeartículo
dc.volumen13
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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