Effect of isoespintanol isolated from Oxandra cf. xylopioides against DNA damage of human lymphocytes
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Date
2018
Journal Title
Journal ISSN
Volume Title
Publisher
UNIV KARACHI
Abstract
Synthetic antioxidants are used in the food and pharmaceutical industry, however, there is concern about their safety; this has prompted the search for new antioxidants that are effective, safe and act at low concentrations. The objective of this study is to evaluate the oxygen radical scavenging capacity and clastogenic effect of the Isoespintanol /2-isopropyl-3,6-dimethyl-5-methylphenol) in DNA of human lymphocyte compared with the BHA (Butylated hydroxyanisole). The oxygen radical scavenging ability was evaluated by methods ORAC(FL) and ORAC(PGR), genotoxicity was determined by comet assay and data analysis was performed using ANOVA and Duncan test. The results show that the oxygen radical scavenging capacity of the BHA is higher than Isoespintanol, however according to the reactivity concept proposed by Lopez-Alarcon and Lissi, the Isoespintanol it is more reactive than BHA. Furthermore, according to some studies, BHA presented adverse effects on the health of consumers. Comet assay results revealed that at concentrations between 3 and 1620 mu M the Isoespintanol don't show clastogenic effects on DNA. In conclusion, the antioxidant capacity for the BHA is higher than Isoespintanol, but considering reactivity concepts proposed by Lopez-Alarcon and Lissi, the Isoespintanol is faster to neutralize radicals that the BHA, furthermore, according to the National Institute of Health "BHA" is a human carcinogen.
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Keywords
DNA damage, antioxidants, BHA, biological products, COMET ASSAY, ANTIOXIDANT PROPERTIES, MICRONUCLEUS TEST, OXIDATIVE DAMAGE, QUERCETIN, SUPPLEMENTATION, FLUORESCEIN, INDUCTION, BEHAVIOR