Revealing the Salmo salar NLRP3 Inflammasome: Insights from Structural Modeling and Transcriptome Analysis

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dc.article.number14556
dc.catalogadorgrr
dc.contributor.authorAcevedo, Waldo
dc.contributor.authorMoran-Figueroa, Rodrigo
dc.contributor.authorVargas-Chacoff, Luis
dc.contributor.authorMorera Galleguillos, Francisco Javier
dc.contributor.authorPontigo, Juan Pablo
dc.date.accessioned2023-11-21T20:30:22Z
dc.date.available2023-11-21T20:30:22Z
dc.date.issued2023
dc.description.abstractThe NLRP3, one of the most heavily studied inflammasome-related proteins in mammals, remains inadequately characterized in Atlantic salmon (Salmo salar), despite the significant commercial importance of this salmonid. The NLRP3 inflammasome is composed of the NLRP3 protein, which is associated with procaspase-1 via an adapter molecule known as ASC. This work aims to characterize the Salmo salar NLRP3 inflammasome through in silico structural modeling, functional transcript expression determination in the SHK-1 cell line in vitro, and a transcriptome analysis on Atlantic salmon. The molecular docking results suggested a similar arrangement of the ternary complex between NLRP3, ASC, and caspase-1 in both the Atlantic salmon and the mammalian NLRP3 inflammasomes. Moreover, the expression results confirmed the functionality of the SsNLRP3 inflammasome in the SHK-1 cells, as evidenced by the lipopolysaccharide-induced increase in the transcription of genes involved in inflammasome activation, including ASC and NLRP3. Additionally, the transcriptome results revealed that most of the inflammasome-related genes, including ASC, NLRP3, and caspase-1, were down-regulated in the Atlantic salmon following its adaptation to seawater (also known as parr-smolt transformation). This is correlated with a temporary detrimental effected on the immune system. Collectively, these findings offer novel insights into the evolutionarily conserved role of NLRP3.
dc.fechaingreso.objetodigital2023-11-21
dc.format.extent18 páginas
dc.fuente.origenWOS
dc.identifier.doi10.3390/ijms241914556
dc.identifier.eissn1422-0067
dc.identifier.issn1661-6596
dc.identifier.urihttps://doi.org/10.3390/ijms241914556
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/75392
dc.identifier.wosidWOS:001084868500001
dc.information.autorucEscuela de Medicina; Morera Galleguillos, Francisco Javier; S/I; 17437
dc.information.autorucEscuela de Ingeniería; Acevedo Castillo, Waldo Andrés; 0000-0002-1182-0253; 219376
dc.issue.numero19
dc.language.isoen
dc.nota.accesoContenido completo
dc.publisherMDPI
dc.revistaInternational Journal of Molecular Sciences
dc.rightsacceso abierto
dc.rights.licenseAtribución 4.0 Internacional (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectNLRP3
dc.subjectInflammasome
dc.subjectInnate immune response
dc.subjectAtlantic salmon
dc.subjectMolecular docking
dc.subjectTranscriptomics
dc.subject.ddc570
dc.subject.deweyBiologíaes_ES
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleRevealing the Salmo salar NLRP3 Inflammasome: Insights from Structural Modeling and Transcriptome Analysis
dc.typeartículo
dc.volumen24
sipa.codpersvinculados17437
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