Betaglycan expression is transcriptionally up-regulated during skeletal muscle differentiation: Cloning of murine betaglycan gene promoter and its modulation by myoD, retinoic acid, and transforming growth factor-beta

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dc.catalogadorpva
dc.contributor.authorLópez-Casillas, Fernando
dc.contributor.authorRiquelme Illanes, Cecilia Angélica
dc.contributor.authorPérez-Kato, Yoshiaki
dc.contributor.authorPonce-Castañeda, M. Verónica
dc.contributor.authorOsses Rivera, Nelson Eduardo
dc.contributor.authorEsparza-López, José
dc.contributor.authorGonzález-Núñez, Gerardo
dc.contributor.authorCabello Verrugio, Claudio Alejandro
dc.contributor.authorMendoza, Valentín
dc.contributor.authorTroncoso, Víctor
dc.contributor.authorBrandan, Enrique
dc.date.accessioned2017-04-11T20:51:22Z
dc.date.available2017-04-11T20:51:22Z
dc.date.issued2003
dc.description.abstractBetaglycan is a membrane-anchored proteoglycan co-receptor that binds transforming growth factor beta (TGF-beta) via its core protein and basic fibroblast growth factor through its glycosaminoglycan chains. In this study we evaluated the expression of betaglycan during the C2C12 skeletal muscle differentiation. Betaglycan expression, as determined by Northern and Western blot, was up-regulated during the conversion of myoblasts to myotubes. The mouse betaglycan gene promoter was cloned, and its sequence showed putative binding sites for SP1, Smad3, Smad4, muscle regulatory factor elements such as MyoD and MEF2, and retinoic acid receptor. Transcriptional activity of the mouse betaglycan promoter reporter was also up-regulated in differentiating C2C12 cells. We found that MyoD, but not myogenin, stimulated this transcriptional activity even in the presence of high serum. Betaglycan promoter activity was increased by RA and inhibited by the three isoforms of TGF-beta. On the other hand, basic fibroblast growth factor, BMP-2, and hepatocyte growth factor/scatter factor, which are inhibitors of myogenesis, had little effect. In myotubes, up-regulated betaglycan was also detectable by TGF-beta affinity labeling and immunofluorescence microscopy studies. The latter indicated that betaglycan was localized both on the cell surface and in the ECM Forced expression of betaglycan in C2C12 myoblasts increases their responsiveness to TGF-beta2, suggesting that it performs a TGF-beta presentation function in this cell lineage. These results indicate that betaglycan expression is up-regulated during myogenesis and that MyoD and RA modulate its expression by a mechanism that is independent of myogenin.
dc.description.funderFONDAP
dc.description.funderMinisterio de Planificación y Cooperación (Chile)
dc.fechaingreso.objetodigital2017-04-11
dc.fuente.origenWOS
dc.identifier.doi10.1074/jbc.M208520200
dc.identifier.eissn1083-351X
dc.identifier.issn0021-9258
dc.identifier.urihttps://doi.org/10.1074/jbc.M208520200
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/18986
dc.information.autorucFacultad de Ciencias Biológicas; Riquelme Illanes, Cecilia Angélica; S/I; 3268
dc.information.autorucFacultad de Ciencias Biológicas; Osses Rivera, Nelson Eduardo; S/I; 3354
dc.information.autorucFacultad de Ciencias Biológicas; Cabello Verrugio, Claudio Alejandro; S/I; 134336
dc.information.autorucFacultad de Ciencias Biológicas; Brandan, Enrique; 0000-0002-6820-5059; 52075
dc.issue.numero1
dc.language.isoen
dc.nota.accesocontenido completo
dc.pagina.final390
dc.pagina.inicio382
dc.publisherElsevier Inc.
dc.revistaJournal of Biological Chemistryes_ES
dc.rightsacceso abierto
dc.rights.licenseAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.subject.otherRegulación de la expresión géneticaes_ES
dc.subject.otherMúsculo esquelético - Crecimiento y desarrolloes_ES
dc.subject.otherProteoglicanoses_ES
dc.titleBetaglycan expression is transcriptionally up-regulated during skeletal muscle differentiation: Cloning of murine betaglycan gene promoter and its modulation by myoD, retinoic acid, and transforming growth factor-beta
dc.typeartículo
dc.volumen278
sipa.codpersvinculados52075
sipa.codpersvinculados3268
sipa.codpersvinculados3354
sipa.codpersvinculados134336
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