Subinhibitory antibiotic concentrations promote the excision of a genomic island carried by the globally spread carbapenem-resistant Klebsiella pneumoniae ST258

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dc.catalogadorgrr
dc.contributor.authorPiña Iturbe, Luis Alejandro
dc.contributor.authorHoppe Elsholz, Guillermo Andres
dc.contributor.authorSuazo Galvez, Isidora Del Carmen
dc.contributor.authorKalergis Parra, Alexis Mikes
dc.contributor.authorBueno Ramírez, Susan
dc.date.accessioned2023-11-24T20:26:49Z
dc.date.available2023-11-24T20:26:49Z
dc.date.issued2023
dc.description.abstractThe ICEKp258.2 genomic island (GI) has been proposed as an important factor for the emergence and success of the globally spread carbapenem-resistant Klebsiella pneumoniae sequence type (ST) 258. However, a characterization of this horizontally acquired element is lacking. Using bioinformatic and experimental approaches, we found that ICEKp258.2 is not confined to ST258 and ST512 but also carried by ST3795 strains and emergent invasive multidrug-resistant pathogens from ST1519. We also identified several ICEKp258.2-like GIs spread among different K. pneumoniae STs, other Klebsiella species, and even other pathogen genera, uncovering horizontal gene transfer events between different STs and bacterial genera. Also, in agreement with the origin of ST258 from ST11, the comparative and phylogenetic analyses of the ICEKp258.2-like GIs suggested that ICEKp258.2 was acquired from an ST11 strain. Importantly, we found that subinhibitory concentrations of antibiotics used in treating K. pneumoniae infections can induce the excision of this GI and modulate its gene expression. Our findings provide the basis for the study of ICEKp258.2 and its role in the success of K. pneumoniae ST258. They also highlight the potential role of antibiotics in the spread of ICEKp258.2-like GIs among bacterial pathogens.
dc.fechaingreso.objetodigital2023-11-24
dc.fuente.origenWOS
dc.identifier.doi10.1101/2023.08.10.552780
dc.identifier.urihttps://doi.org/10.1101/2023.08.10.552780
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/75418
dc.identifier.wosidPPRN:75237904
dc.information.autorucFacultad de Ciencias Biológicas; Piña Iturbe, Luis Alejandro; 0000-0001-6190-9494; 1031369
dc.information.autorucFacultad de Ciencias Biológicas; Hoppe Elsholz, Guillermo Andres; 0000-0003-2029-9700; 1047211
dc.information.autorucFacultad de Ciencias Biológicas; Suazo Galvez, Isidora Del Carmen; 0000-0001-8274-7471; 215403
dc.information.autorucFacultad de Ciencias Biológicas; Kalergis Parra, Alexis Mikes; 0000-0001-7622-5263; 90610
dc.information.autorucFacultad de Ciencias Biológicas; Bueno Ramírez, Susan; 0000-0002-7551-8088; 113541
dc.language.isoen
dc.nota.accesoContenido completo
dc.publisherCold Spring HarborLab. Press, Publications Dept.
dc.revistabioRxiv
dc.rightsacceso abierto
dc.rights.licenseAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)
dc.subject.ddc520
dc.subject.deweyAstronomíaes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleSubinhibitory antibiotic concentrations promote the excision of a genomic island carried by the globally spread carbapenem-resistant Klebsiella pneumoniae ST258
dc.typepreprint
sipa.codpersvinculados1031369
sipa.codpersvinculados1047211
sipa.codpersvinculados215403
sipa.codpersvinculados90610
sipa.codpersvinculados113541
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