Porin alterations present in non-carbapenemase-producing Enterobacteriaceae with high and intermediate levels of carbapenem resistance in Chile

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dc.contributor.authorWozniak, Aniela
dc.contributor.authorVillagra, Nicolas A.
dc.contributor.authorUndabarrena, Agustina
dc.contributor.authorGallardo, Natalia
dc.contributor.authorKeller, Nicole
dc.contributor.authorMoraga, Marcela
dc.contributor.authorRoman, Juan C.
dc.contributor.authorMora, Guido C.
dc.contributor.authorGarcia, Patricia
dc.date.accessioned2024-01-10T13:16:22Z
dc.date.available2024-01-10T13:16:22Z
dc.date.issued2012
dc.description.abstractThe main goal of this work was to identify the mechanisms responsible for carbapenem resistance in 61 Chilean clinical isolates of Enterobacteriaceae (Enterobacter spp., Serratia marcescens, Morganella morganii, Escherichia coli and Klebsiella pneumoniae) with reduced susceptibility to at least one carbapenem (ertapenem, imipenem or meropenem). All of the isolates were analysed for the presence of carbapenemases, extended spectrum beta-lactamases (ESBLs), AmpC enzymes and outer-membrane proteins. None of the isolates exhibited carbapenemase activity nor did they have any of the carbapenemase genes that were screened for. Most of the 61 strains produced at least one ESBL and/or one AmpC enzyme and either lost their porins or had altered porins according to sequence analysis. The distribution of ESBLs and AmpC enzymes was different among the species studied. Resistance in K. pneumoniae and E. coli isolates was associated with ESBLs; in M. morganii isolates, resistance was attributed to overexpression of an AmpC enzyme; and in Enterobacter spp. isolates, resistance was associated with both types of enzymes. In K. pneumoniae isolates, porin integrity was more a determinant of carbapenem resistance than the presence of ESBLs, whereas in isolates of Enterobacter spp., M. morganii and S. marcescens, the presence of an overexpressed AmpC enzyme was associated with higher imipenem and meropenem MIC values. Therefore, carbapenem resistance in Chilean isolates is not due to true carbapenemases but rather to a combination of porin loss/alteration and beta-lactamase activity. The fact that carbapenemases were not detected in this study is unique, given that many countries in the region have already reported the presence of these enzymes.
dc.description.funderFondos Departamentales Concursables - Departamento de Laboratorios Clinicos
dc.description.funderSentry Program
dc.fechaingreso.objetodigital2024-05-23
dc.format.extent10 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1099/jmm.0.045799-0
dc.identifier.issn0022-2615
dc.identifier.pubmedidMEDLINE:22700549
dc.identifier.urihttps://doi.org/10.1099/jmm.0.045799-0
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/78579
dc.identifier.wosidWOS:000309193900011
dc.information.autorucMedicina;Gallardo N;S/I;156245
dc.information.autorucMedicina;Garcia P;S/I;73909
dc.information.autorucMedicina;Keller N;S/I;156268
dc.information.autorucMedicina;Moraga M;S/I;1007403
dc.information.autorucMedicina;Roman J;S/I;117336
dc.information.autorucMedicina;Undabarrena A;S/I;163485
dc.information.autorucMedicina;Wozniak A;S/I;1008612
dc.issue.numero9
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final1279
dc.pagina.inicio1270
dc.publisherSOC GENERAL MICROBIOLOGY
dc.revistaJOURNAL OF MEDICAL MICROBIOLOGY
dc.rightsacceso restringido
dc.subjectPENICILLIN-BINDING PROTEINS
dc.subjectAMPC BETA-LACTAMASES
dc.subjectKLEBSIELLA-PNEUMONIAE
dc.subjectESCHERICHIA-COLI
dc.subjectSTAPHYLOCOCCUS-AUREUS
dc.subjectOUTER-MEMBRANE
dc.subjectSOUTH-AMERICA
dc.subjectINFECTIONS
dc.subjectKPC-2
dc.subjectMORGANELLA
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titlePorin alterations present in non-carbapenemase-producing Enterobacteriaceae with high and intermediate levels of carbapenem resistance in Chile
dc.typeartículo
dc.volumen61
sipa.codpersvinculados156245
sipa.codpersvinculados73909
sipa.codpersvinculados156268
sipa.codpersvinculados1007403
sipa.codpersvinculados117336
sipa.codpersvinculados163485
sipa.codpersvinculados1008612
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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