Proinflammatory cytokine expression in gastric tissue from children with Helicobacter pylori-associated gastritis
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Date
2001
Journal Title
Journal ISSN
Volume Title
Publisher
LIPPINCOTT WILLIAMS & WILKINS
Abstract
Background: Helicobacter pylori infection of the gastric mucosa in humans is usually acquired early in life. The chronic inflammation that ensues involves the increased production of inflammatory cytokines. Published data on production of these mediators by gastric mucosa of H. pylori-infected children are few.
Methods: Seventy-nine children, aged 5 to 18 years, referred for upper gastrointestinal endoscopy to four separate hospitals in Chile, were studied. The concentrations of interleukin (IL)-1 beta, IL-6, IL-8, and tumor necrosis factor a were measured in homogenates of gastric mucosal biopsy specimens. Cytokine expression was confirmed by reverse transcription polymerase chain reaction. These data were correlated with the patients' clinical, histologic and sociodemographic status.
Results: Patient rate of colonization by H. pylori was inversely correlated with socioeconomic status (P < 0.005) and positively correlated with age (P < 0.0025). In gastric mucosa, concentrations of IL-1 beta, IL-8, and tumor necrosis factor a were all significantly higher in H. pylori-positive patients than in H. pylori-negative patients and in patients who had histologic gastritis than in those with normal gastric mucosa. In patients with peptic ulcer disease, only IL-1 beta and IL-8 concentrations were significantly elevated when compared with those of patients without ulcers. Interleukin-6 concentrations were comparable among the different groups analyzed.
Conclusions: This study suggests that increased gastric mucosal production of the proinflammatory cytokines IL-1 beta and IL-8 is probably involved in H. pylori-associated gastric damage in children and may be crucial in determining the different clinical outcomes.
Methods: Seventy-nine children, aged 5 to 18 years, referred for upper gastrointestinal endoscopy to four separate hospitals in Chile, were studied. The concentrations of interleukin (IL)-1 beta, IL-6, IL-8, and tumor necrosis factor a were measured in homogenates of gastric mucosal biopsy specimens. Cytokine expression was confirmed by reverse transcription polymerase chain reaction. These data were correlated with the patients' clinical, histologic and sociodemographic status.
Results: Patient rate of colonization by H. pylori was inversely correlated with socioeconomic status (P < 0.005) and positively correlated with age (P < 0.0025). In gastric mucosa, concentrations of IL-1 beta, IL-8, and tumor necrosis factor a were all significantly higher in H. pylori-positive patients than in H. pylori-negative patients and in patients who had histologic gastritis than in those with normal gastric mucosa. In patients with peptic ulcer disease, only IL-1 beta and IL-8 concentrations were significantly elevated when compared with those of patients without ulcers. Interleukin-6 concentrations were comparable among the different groups analyzed.
Conclusions: This study suggests that increased gastric mucosal production of the proinflammatory cytokines IL-1 beta and IL-8 is probably involved in H. pylori-associated gastric damage in children and may be crucial in determining the different clinical outcomes.
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Keywords
Helicobacter pylori, cytokines, gastritis, children, peptic ulcer, TUMOR-NECROSIS-FACTOR, SURFACE-PROTEINS, FACTOR-ALPHA, MACROPHAGES, INFECTION, RNA, INTERLEUKIN-8, EPIDEMIOLOGY, IMMUNITY, UREASE