Thymic stromal lymphopoietin participates in the host response to intra-amniotic inflammation leading to preterm labor and birth

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dc.contributor.authorKanninen, Tomi
dc.contributor.authorTao, Li
dc.contributor.authorRomero, Roberto
dc.contributor.authorXu, Yi
dc.contributor.author-Hernandez, Marcia Arenas
dc.contributor.authorGalaz, Jose
dc.contributor.authorLiu, Zhenjie
dc.contributor.authorMiller, Derek
dc.contributor.authorLevenson, Dustyn
dc.contributor.authorGreenberg, Jonathan M.
dc.contributor.authorPanzer, Jonathan
dc.contributor.authorPadron, Justin
dc.contributor.authorTheis, Kevin R.
dc.contributor.authorGomez-Lopez, Nardhy
dc.date.accessioned2025-01-20T20:06:03Z
dc.date.available2025-01-20T20:06:03Z
dc.date.issued2023
dc.description.abstractThe aim of this study was to establish the role of thymic stromal lymphopoietin (TSLP) in the intra-amniotic host response of women with spontaneous preterm labor (sPTL) and birth. Amniotic fluid and chorioamniotic membranes (CAM) were collected from women with sPTL who delivered at term (n = 30) or preterm without intra-amniotic inflammation (n = 34), with sterile intra-amniotic inflammation (SIAI, n = 27), or with intra-amniotic infection (IAI, n = 17). Amnion epithelial cells (AEC), Ureaplasma parvum, and Sneathia spp. were also utilized. The expression of TSLP, TSLPR, and IL-7R & alpha; was evaluated in amniotic fluid or CAM by RT-qPCR and/or immunoassays. AEC co-cultured with Ureaplasma parvum or Sneathia spp. were evaluated for TSLP expression by immunofluorescence and/or RT-qPCR. Our data show that TSLP was elevated in amniotic fluid of women with SIAI or IAI and expressed by the CAM. TSLPR and IL-7R & alpha; had detectable gene and protein expression in the CAM; yet, CRLF2 was specifically elevated with IAI. While TSLP localized to all layers of the CAM and increased with SIAI or IAI, TSLPR and IL-7R & alpha; were minimal and became most apparent with IAI. Co -culture experiments indicated that Ureaplasma parvum and Sneathia spp. differentially upregulated TSLP expression in AEC. Together, these findings indicate that TSLP is a central component of the intra-amniotic host response during sPTL.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.humimm.2023.06.005
dc.identifier.eissn1879-1166
dc.identifier.issn0198-8859
dc.identifier.urihttps://doi.org/10.1016/j.humimm.2023.06.005
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/91730
dc.identifier.wosidWOS:001064679400001
dc.issue.numero9
dc.language.isoen
dc.pagina.final463
dc.pagina.inicio450
dc.revistaHuman immunology
dc.rightsacceso restringido
dc.subjectAmniotic fluid
dc.subjectChorioamniotic membranes
dc.subjectSneathia
dc.subjectTSLP
dc.subjectUreaplasma
dc.subject.ods05 Gender Equality
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa05 Igualdad de género
dc.subject.odspa03 Salud y bienestar
dc.titleThymic stromal lymphopoietin participates in the host response to intra-amniotic inflammation leading to preterm labor and birth
dc.typeartículo
dc.volumen84
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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