Downregulation of exosomal miR-192-5p and miR-204-5p in subjects with nonclassic apparent mineralocorticoid excess.

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dc.contributor.authorTapia Castillo, Alejandra.
dc.contributor.authorBarros, Eric.
dc.contributor.authorAllende, Fidel
dc.contributor.authorVecchiola Cárdenas, Andrea Paola
dc.contributor.authorFardella B., Carlos
dc.contributor.authorCarvajal Maldonado, Cristián Andrés
dc.contributor.authorGuanzon, Dominic.
dc.contributor.authorPalma, Carlos.
dc.contributor.authorLai, Andrew.
dc.contributor.authorSalomón Gallo, Carlos Francisco.
dc.date.accessioned2019-12-10T14:35:38Z
dc.date.available2019-12-10T14:35:38Z
dc.date.issued2019
dc.date.updated2019-12-01T01:02:20Z
dc.description.abstractAbstract Background The “nonclassic” apparent mineralocorticoid excess (NC-AME) has been identified in approximately 7% of general population. This phenotype is characterized by low plasma renin activity (PRA), high serum cortisol (F) to cortisone (E) ratio, low cortisone, high Fractional Excretion of potassium (FEK) and normal-elevated systolic blood pressure (SBP). An early detection and/or identification of novel biomarkers of this phenotype could avoid the progression or future complications leading to arterial hypertension. Isolation of extracellular vesicles, such as exosomes, in specific biofluids support the identification of tissue-specific RNA and miRNA, which may be useful as novel biomarkers. Our aim was to identify miRNAs within urinary exosomes associated to the NC-AME phenotype. Methods We perform a cross-sectional study in a primary care cohort of 127 Chilean subjects. We measured BP, serum cortisol, cortisone, aldosterone, PRA. According to the previous reported, a subgroup of subjects was classified as NC-AME (n = 10). Urinary exosomes were isolated and miRNA cargo was sequenced by Illumina-NextSeq-500. Results We found that NC-AME subjects had lower cortisone (p < 0.0001), higher F/E ratio (p < 0.0001), lower serum potassium (p = 0.009) and higher FEK 24 h (p = 0.03) than controls. We found miR-204-5p (fold-change = 0.115; p 0.001) and miR-192-5p (fold-change = 0.246; p 0.03) are both significantly downregulated in NC-AME. miR-192-5p expression was correlated with PRA (r = 0.45; p 0.028) and miR-204-5p expression with SBP (r = − 0.48, p 0.027) and F/E ratio (r = − 0.48; p 0.026). Conclusions These findings could support a potential role of these miRNAs as regulators and novel biomarkers of the NC-AME phenotype.
dc.fuente.origenBiomed Central
dc.identifier.citationJournal of Translational Medicine. 2019 Nov 27;17(1):392
dc.identifier.doi10.1186/s12967-019-02143-8
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/26996
dc.issue.numeroNo. 392
dc.pagina.final11
dc.pagina.inicio1
dc.revistaJournal of Translational Medicinees_ES
dc.rightsacceso abierto
dc.rights.holderThe Author(s)
dc.subjectNonclassic AMEes_ES
dc.subjectMineralocorticoid receptores_ES
dc.subjectMicroRNAes_ES
dc.subjectExosomeses_ES
dc.subjectHypertensiones_ES
dc.subject.ddc616.132
dc.subject.deweyMedicina y saludes_ES
dc.titleDownregulation of exosomal miR-192-5p and miR-204-5p in subjects with nonclassic apparent mineralocorticoid excess.es_ES
dc.typeartículo
dc.volumenVol. 17
sipa.codpersvinculados1030648
sipa.codpersvinculados1004550
sipa.codpersvinculados123319
sipa.codpersvinculados66235
sipa.codpersvinculados8586
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