Transforming growth factor-beta 1 produced by hippocampal cells modulates microglial reactivity in culture

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dc.contributor.authorHerrera Molina, R
dc.contributor.authorvon Bernhardi, R
dc.date.accessioned2024-01-10T13:44:01Z
dc.date.available2024-01-10T13:44:01Z
dc.date.issued2005
dc.description.abstractActivated microglia produce superoxide anion (O-2(center dot-)) and nitric oxide (NO), both of which can be neurotoxic. To identify regulatory mechanisms that might modulate over-activation of microglia, we evaluated the inhibition of microglial activation by factors secreted by hippocampal cells. Supernatants from hippocampal cell cultures (Hippocampal-Cm) prevented microglial O-2(center dot) and NO production. LAP-TGF beta 1 was present in the Hippocampal-Cm as shown by immunoblot and a TGF beta 1-dependent proliferation-inhibition bioassay. LAP-TGF beta 1 and TGF beta activity increased in hippocampal cultures exposed to proinflammatory conditions (LPS and Interferon-gamma). The inhibition of (O-2(center dot-)) and NO production by Hippocampal-Cm was mimicked by the addition of recombinant TGF beta 1. Treating Hippocampal-Cm with an antibody against TGF beta 1 to neutralize its activity eliminated its ability to inhibit O-2(center dot-) and NO production. Our findings suggest that the TGF beta 1 secreted by hippocampal cells modulated microglial activity. We propose that in pathological conditions, impairment of this modulatory mechanism could enhance microglia-mediated neurotoxicity. (c) 2005 Elsevier Inc. All rights reserved.
dc.fechaingreso.objetodigital03-04-2024
dc.format.extent8 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.nbd.2005.01.003
dc.identifier.eissn1095-953X
dc.identifier.issn0969-9961
dc.identifier.pubmedidMEDLINE:15837578
dc.identifier.urihttps://doi.org/10.1016/j.nbd.2005.01.003
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/78819
dc.identifier.wosidWOS:000228672900024
dc.information.autorucMedicina;Von Bernhardi R;S/I;62523
dc.issue.numero1-2
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final236
dc.pagina.inicio229
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCE
dc.revistaNEUROBIOLOGY OF DISEASE
dc.rightsacceso restringido
dc.subjectcytokines
dc.subjecthippocampal cell
dc.subjectinflammation
dc.subjectmicroglial cells
dc.subjectneurodegeneration
dc.subjectnitric oxide
dc.subjectsuperoxide anion
dc.subjectTGF beta 1
dc.subjectNITRIC-OXIDE SYNTHASE
dc.subjectINFLAMMATORY RESPONSE
dc.subjectTGF-BETA
dc.subjectOXIDATIVE STRESS
dc.subjectBRAIN-INJURY
dc.subjectIFN-GAMMA
dc.subjectNEURONS
dc.subjectACTIVATION
dc.subjectEXPRESSION
dc.subjectTGF-BETA-1
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleTransforming growth factor-beta 1 produced by hippocampal cells modulates microglial reactivity in culture
dc.typeartículo
dc.volumen19
sipa.codpersvinculados62523
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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