Molecular MR-Imaging for Noninvasive Quantification of the Anti-Inflammatory Effect of Targeting Interleukin-1β in a Mouse Model of Aortic Aneurysm

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dc.catalogadorvzp
dc.contributor.authorBrangsch, Julia
dc.contributor.authorReimann, Carolin
dc.contributor.authorKaufmann, Jan Ole
dc.contributor.authorAdams, Lisa Christine
dc.contributor.authorHamm, Bernd
dc.contributor.authorMakowski, Marcus Richard
dc.contributor.authorThöne-Reineke, Christa
dc.contributor.authorWilke, Marco
dc.contributor.authorWeller, Michael
dc.contributor.authorOnthank, David
dc.contributor.authorRobinson, Simon
dc.contributor.authorBuchholz, Rebecca
dc.contributor.authorKarst, Uwe
dc.contributor.authorBotnar, Rene Michael
dc.date.accessioned2024-01-22T14:50:52Z
dc.date.available2024-01-22T14:50:52Z
dc.date.issued2020
dc.description.abstractMolecular-MRI is a promising imaging modality for the assessment of abdominal aortic aneurysms (AAAs). Interleukin-1β (IL-1β) represents a new therapeutic tool for AAA-treatment, since pro-inflammatory cytokines are key-mediators of inflammation. This study investigates the potential of molecular-MRI to evaluate therapeutic effects of an anti-IL-1β-therapy on AAA-formation in a mouse-model. Methods: Osmotic-minipumps were implanted in apolipoprotein-deficient-mice (N = 27). One group (Ang-II+01BSUR group, n = 9) was infused with angiotensin-II (Ang-II) for 4 weeks and received an anti-murine IL-1β-antibody (01BSUR) 3 times. One group (Ang-II-group, n = 9) was infused with Ang-II for 4 weeks but received no treatment. Control-group (n = 9) was infused with saline and received no treatment. MR-imaging was performed using an elastin-specific gadolinium-based-probe (0.2 mmol/kg). Results: Mice of the Ang-II+01BSUR-group showed a lower aortic-diameter compared to mice of the Ang-II-group and control mice (p < 0.05). Using the elastin-specific-probe, a significant decrease in elastin-destruction was observed in mice of the Ang-II+01BSUR-group. In vivo MR-measurements correlated well with histopathology (y = 0.34x-13.81, R2 = 0.84, p < 0.05), ICP-MS (y = 0.02x+2.39; R2 = 0.81, p < 0.05) and LA-ICP-MS. Immunofluorescence and western-blotting confirmed a reduced IL-1β-expression. Conclusions: Molecular-MRI enables the early visualization and quantification of the anti-inflammatory-effects of an IL-1β-inhibitor in a mouse-model of AAAs. Responders and non-responders could be identified early after the initiation of the therapy using molecular-MRI.
dc.description.funderKing?s College London
dc.description.funderMass Spectrometry Facility
dc.description.funderNovartis
dc.description.funderLantheus Medical Imaging
dc.description.funderKing’s College London
dc.description.funderWellcome Trust
dc.description.funderBHF
dc.description.funderDeutsche Forschungsgemeinschaft
dc.description.funderCharité–Universitaetsmedizin Berlin
dc.description.funderBerlin Institute of Health
dc.fuente.origenScopus
dc.identifier.doi10.1177/1536012120961875
dc.identifier.eisbn1536-0121
dc.identifier.issn1535-3508
dc.identifier.pubmedidMEDLINE:33216687
dc.identifier.scopusidSCOPUS_ID:85096335825
dc.identifier.urihttp://mix.sagepub.com/content/by/year
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/80871
dc.identifier.wosidWOS:000593576400001
dc.information.autorucInstituto de Ingeniería Biológica y Médica; Botnar Rene Michael; 0000-0002-9447-4367; 1015313
dc.language.isoen
dc.nota.accesoContenido completo
dc.publisherSAGE Publications Inc.
dc.revistaMolecular Imaging
dc.rights.licenseCreative Commons Attribution-NonCommercial License (BY-NC-4.0)
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subjectAnimal models of disease
dc.subjectCardiovascular
dc.subject.ddc610
dc.subject.deweyMedicina y salud
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleMolecular MR-Imaging for Noninvasive Quantification of the Anti-Inflammatory Effect of Targeting Interleukin-1β in a Mouse Model of Aortic Aneurysm
dc.typeartículo
dc.volumen19
sipa.codpersvinculados1015313
sipa.trazabilidadScopus;12-10-2021
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