Anti-Apoptotic Effects of 3,3’,5-Triiodo-L-Thyronine in the Liver of Brain-Dead Rats

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dc.article.numbere0138749
dc.contributor.authorRebolledo Acevedo, Rolando Arturo
dc.contributor.authorVan Erp, Anne C.
dc.contributor.authorOttens, Petra J.
dc.contributor.authorWiersema-Buist, Janneke
dc.contributor.authorLeuvenink, Henri G. D.
dc.contributor.authorRomanque, Pamela
dc.date.accessioned2023-01-13T19:14:43Z
dc.date.available2023-01-13T19:14:43Z
dc.date.issued2015
dc.description.abstractBackground: Thyroid hormone treatment in brain-dead organ donors has been extensively studied and applied in the clinical setting. However, its clinical applicability remains controversial due to a varying degree of success and a lack of mechanistic understanding about the therapeutic effects of 3,3’,5-Triiodo-L-thyronine (T3). T3 pre-conditioning leads to anti-apoptotic and pro-mitotic effects in liver tissue following ischemia/reperfusion injury. Therefore, we aimed to study the effects of T3 pre-conditioning in the liver of brain-dead rats. Methods: Brain death (BD) was induced in mechanically ventilated rats by inflation of a Fogarty catheter in the epidural space. T3 (0.1 mg/kg) or vehicle was administered intraperitoneally 2 h prior to BD induction. After 4 h of BD, serum and liver tissue were collected. RT-qPCR, routine biochemistry, and immunohistochemistry were performed. Results: Brain-dead animals treated with T3 had lower plasma levels of AST and ALT, reduced Bax gene expression, and less hepatic cleaved Caspase-3 activation compared to brain-dead animals treated with vehicle. Interestingly, no differences in the expression of inflammatory genes (IL-6, MCP-1, IL-1β) or the presence of pro-mitotic markers (Cyclin-D and Ki-67) were found in brain-dead animals treated with T3 compared to vehicle-treated animals. Conclusion: T3 pre-conditioning leads to beneficial effects in the liver of brain-dead rats as seen by lower cellular injury and reduced apoptosis, and supports the suggested role of T3 hormone therapy in the management of brain-dead donors.
dc.fuente.origenSIPA
dc.identifier.doi10.1371/journal.pone.0138749
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0138749
dc.identifier.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0138749
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/66306
dc.information.autorucEscuela de medicina ; Rebolledo Acevedo, Rolando Arturo ; 0000-0003-3501-4042 ; 127520
dc.language.isoen
dc.nota.accesoContenido completo
dc.revistaPLoS ONEes_ES
dc.rightsacceso abierto
dc.titleAnti-Apoptotic Effects of 3,3’,5-Triiodo-L-Thyronine in the Liver of Brain-Dead Ratses_ES
dc.typeartículo
sipa.codpersvinculados127520
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