Revealing RCOR2 as a regulatory component of nuclear speckles

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dc.article.number51
dc.contributor.authorRivera Álvarez, Carlos Andrés
dc.contributor.authorVerbel Vergara, Daniel Eduardo
dc.contributor.authorArancibia Radich, Duxan Andrés
dc.contributor.authorLappala, Anna
dc.contributor.authorGonzález, Marcela
dc.contributor.authorGuzmán, Fabián
dc.contributor.authorMerello, Gianluca
dc.contributor.authorLee, Jeannie T.
dc.contributor.authorAndrés Coke, María Estela
dc.date.accessioned2021-12-17T20:30:36Z
dc.date.available2021-12-17T20:30:36Z
dc.date.issued2021
dc.date.updated2021-11-28T01:02:12Z
dc.description.abstractBackground: Nuclear processes such as transcription and RNA maturation can be impacted by subnuclear compartmentalization in condensates and nuclear bodies. Here, we characterize the nature of nuclear granules formed by REST corepressor 2 (RCOR2), a nuclear protein essential for pluripotency maintenance and central nervous system development. Results: Using biochemical approaches and high-resolution microscopy, we reveal that RCOR2 is localized in nuclear speckles across multiple cell types, including neurons in the brain. RCOR2 forms complexes with nuclear speckle components such as SON, SRSF7, and SRRM2. When cells are exposed to chemical stress, RCOR2 behaves as a core component of the nuclear speckle and is stabilized by RNA. In turn, nuclear speckle morphology appears to depend on RCOR2. Specifically, RCOR2 knockdown results larger nuclear speckles, whereas overexpressing RCOR2 leads to smaller and rounder nuclear speckles. Conclusion: Our study suggests that RCOR2 is a regulatory component of the nuclear speckle bodies, setting this co-repressor protein as a factor that controls nuclear speckles behavior.
dc.format.extent15 páginas
dc.fuente.origenAutoarchivo
dc.identifier.citationEpigenetics & Chromatin. 2021 Nov 24;14(1):51
dc.identifier.doi10.1186/s13072-021-00425-4
dc.identifier.urihttps://doi.org/10.1186/s13072-021-00425-4
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/63059
dc.information.autorucFacultad de Ciencias Biológicas ; Rivera Álvarez, Carlos Andrés ; 0000-0001-5326-4139 ; 1015392
dc.information.autorucFacultad de Ciencias Biológicas ; Verbel Vergara, Daniel Eduardo ; 0000-0002-7186-6413 ; 1070752
dc.information.autorucFacultad de Ciencias Biológicas ; Arancibia Radich, Duxan Andrés ; 0000-0003-4423-5429 ; 250241
dc.information.autorucFacultad de Ciencias Biológicas ; González, Marcela ; S/I ; 1011022
dc.information.autorucFacultad de Ciencias Biológicas ; Guzmán, Fabián ; S/I ; 0
dc.information.autorucFacultad de Ciencias Biológicas ; Merello, Gianluca ; 0000-0002-3317-6198 ; 0
dc.information.autorucFacultad de Ciencias Biológicas ; Andrés Coke, María Estela ; 74858
dc.language.isoen
dc.nota.accesoContenido completo
dc.pagina.final15
dc.pagina.inicio1
dc.revistaEpigenetics & Chromatin
dc.rightsacceso abierto
dc.rights.holderThe Author(s)
dc.subjectCoRESTes_ES
dc.subjectCoREST2es_ES
dc.subjectRCOR2es_ES
dc.subjectNuclear speckleses_ES
dc.subjectMembrane-less organelleses_ES
dc.subjectSRRM2es_ES
dc.subjectSRSF7es_ES
dc.subjectSC35es_ES
dc.subjectSRSF2es_ES
dc.subjectSONes_ES
dc.subject.ddc570
dc.subject.deweyBiologíaes_ES
dc.titleRevealing RCOR2 as a regulatory component of nuclear speckleses_ES
dc.typeartículo
dc.volumen14
sipa.codpersvinculados1015392
sipa.codpersvinculados1070752
sipa.codpersvinculados250241
sipa.codpersvinculados1011022
sipa.codpersvinculados74858
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