Progesterone Utilizes Distinct Membrane Pools of Tissue Factor to Increase Coagulation and Invasion and These Effects are Inhibited by TFPI

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dc.contributor.authorHenriquez, Soledad
dc.contributor.authorCalderon, Claudia
dc.contributor.authorQuezada, Marisol
dc.contributor.authorOliva, Barbara
dc.contributor.authorLoreto Bravo, Maria
dc.contributor.authorAranda, Evelyn
dc.contributor.authorKato, Sumie
dc.contributor.authorCuello, Mauricio A.
dc.contributor.authorGutierrez, Jorge
dc.contributor.authorQuest, Andrew F. G.
dc.contributor.authorOwen, Gareth I.
dc.date.accessioned2024-01-10T13:16:41Z
dc.date.available2024-01-10T13:16:41Z
dc.date.issued2011
dc.description.abstractTissue factor (TF) serving as the receptor for coagulation factor VII (FVII) initiates the extrinsic coagulation pathway. We previously demonstrated that progesterone increases TF, coagulation and invasion in breast cancer cell lines. Herein, we investigated if tissue factor pathway inhibitor (TFPI) could down-regulate progesterone-increased TF activity in these cells. Classically, TFPI redistributes TF-FVII-FX-TFPI in an inactive quaternary complex to membrane associated lipid raft regions. Herein, we demonstrate that TF increased by progesterone is localized to the heavy membrane fraction, despite progesterone-increased coagulation originating almost exclusively from lipid raft domains, where TF levels are extremely low. The progesterone increase in coagulation is not a rapid effect, but is progesterone receptor (PR) dependent and requires protein synthesis. Although a partial relocalization of TF occurs, TFPI does not require the redistribution to lipid rafts to inhibit coagulation or invasion. Inhibition by TFPI and anti-TF antibodies in lipid raft membrane fractions confirmed the dependence on TF for progesterone-mediated coagulation. Through the use of pathway inhibitors, we further demonstrate that the TF up-regulated by progesterone is not coupled to the progesterone increase in TF-mediated coagulation. However, the progesterone up-regulated TF protein may be involved in progesterone-mediated breast cancer cell invasion, which TFPI also inhibits. J. Cell. Physiol. 226: 3278-3285, 2011. (C) 2011 Wiley Periodicals, Inc.
dc.description.funderChilean Fondo Nacional de Desarrollo Cientifico y Tecnologico
dc.description.funderFondo de Fomento al Desarrollo Cientifico y Tecnologico
dc.description.funderFondo de Investigacion Avanzada en Areas Prioritarias
dc.fechaingreso.objetodigital2024-04-27
dc.format.extent8 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1002/jcp.22689
dc.identifier.eissn1097-4652
dc.identifier.issn0021-9541
dc.identifier.pubmedidMEDLINE:21344386
dc.identifier.urihttps://doi.org/10.1002/jcp.22689
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/78605
dc.identifier.wosidWOS:000296458100021
dc.information.autorucCiencias Biológicas;Aranda E ;S/I;15771
dc.information.autorucCiencias Biológicas;Bravo M;S/I;125561
dc.information.autorucMedicina;Cuello M;S/I;80064
dc.information.autorucCiencias Biológicas;Henriquez S ;S/I;158185
dc.information.autorucMedicina;Kato S ;S/I;82640
dc.information.autorucCiencias Biológicas;Oliva B ;S/I;1008557
dc.information.autorucCiencias Biológicas;Owen G;S/I;1000459
dc.information.autorucCiencias Biológicas;Quezada M ;S/I;158184
dc.issue.numero12
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.final3285
dc.pagina.inicio3278
dc.publisherWILEY
dc.revistaJOURNAL OF CELLULAR PHYSIOLOGY
dc.rightsacceso restringido
dc.subjectFACTOR PATHWAY INHIBITOR
dc.subjectFACTOR PROCOAGULANT ACTIVITY
dc.subjectFACTOR EXPRESSION
dc.subjectDE-ENCRYPTION
dc.subjectCELL-SURFACE
dc.subjectTUMOR-GROWTH
dc.subjectCANCER
dc.subjectMECHANISM
dc.subjectCAVEOLAE
dc.subjectPROTEIN
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleProgesterone Utilizes Distinct Membrane Pools of Tissue Factor to Increase Coagulation and Invasion and These Effects are Inhibited by TFPI
dc.typeartículo
dc.volumen226
sipa.codpersvinculados15771
sipa.codpersvinculados125561
sipa.codpersvinculados80064
sipa.codpersvinculados158185
sipa.codpersvinculados82640
sipa.codpersvinculados1008557
sipa.codpersvinculados1000459
sipa.codpersvinculados158184
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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