Spontaneous formation in air of DPPC Supported Lipid Bilayers (SLBs) evaporated in a solvent free process on silicon substrates

Simple item page
dc.contributor.advisorVolkmann, Ulrich
dc.contributor.authorCisternas Fruns, Marcelo Andrés
dc.contributor.otherPontificia Universidad Católica de Chile. Instituto de Física
dc.date.accessioned2021-06-11T13:41:17Z
dc.date.available2021-06-11T13:41:17Z
dc.date.issued2021
dc.date.updated2021-06-07T00:22:36Z
dc.descriptionTesis (Doctor en Física)--Pontificia Universidad Católica de Chile, 2021
dc.description.abstractArtificial membranes are models for biological systems and are important to gain deeper insight into biological membranes and for various applications. We introduce a dry two-step self-assembly method consisting of the high-vacuum evaporation of phospholipid molecules over silicon, followed by a subsequent annealing step in air. We evaporate dipalmitoylphosphatidylcholine (DPPC) molecules over bare silicon without the use of polymer cushions or solvents. High-resolution ellipsometry and AFM temperature-dependent measurements are performed in air to detect the characteristic phase transitions of DPPC bilayers. Complementary AFM force-spectroscopy breakthrough events are induced to detect single- and multi-bilayer formations. These combined experimental methods confirm the formation of stable non-hydrated lipid bilayers with phase transitions between gel to ripple phases at 311.5 ± 0.9 K, ripple to liquid crystalline phases at 323.8 ± 2.5 K and liquid crystalline to fluid disordered phases at 330.4 ± 0.9 K, which was consistent with such structures reported in wet environments. We find that the AFM tip induces a restructuring or intercalation of the bilayer that is strongly related to the applied tip-force. These dry supported lipid bilayers show long-term stability. These findings are relevant for the development of functional biointerfaces, specifically for fabrication of biosensors and membrane protein platforms. The observed stability is relevant in the context of lifetimes of such systems protected by bilayers in dry environments, such as e.g. SARS-CoV-2 virus.
dc.format.extent129 páginas
dc.fuente.origenAutoarchivo
dc.identifier.doi10.7764/tesisUC/FIS/60584
dc.identifier.urihttps://doi.org/10.7764/tesisUC/FIS/60584
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/60584
dc.information.autorucInstituto de Física ; Volkmann, Ulrich, 1958- ; 0000-0003-4972-5558 ; 100470
dc.information.autorucInstituto de Física ; Cisternas Fruns, Marcelo Andrés ; 0000-0002-8612-8560 ; 148226
dc.language.isoen
dc.nota.accesoContenido completo
dc.rightsacceso abierto
dc.subject.ddc571.64
dc.subject.deweyBiologíaes_ES
dc.subject.otherMembranas artificialeses_ES
dc.subject.otherElipsometríaes_ES
dc.subject.otherBiosensoreses_ES
dc.subject.otherMicroscopía de fuerza atómicaes_ES
dc.titleSpontaneous formation in air of DPPC Supported Lipid Bilayers (SLBs) evaporated in a solvent free process on silicon substrateses_ES
dc.typetesis doctoral
sipa.codpersvinculados100470
sipa.codpersvinculados148226
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Thesis MCisternas Junio 2021_Final Version 2.pdf
Size:
5.39 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
0 B
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
3.10 Tesis doctorado