Cross-sectional comparative shotgun metagenomic analysis of the subgingival resistome in healthy subjects and patients with periodontitis from four countries

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dc.catalogadorjlo
dc.contributor.authorArredondo, A.
dc.contributor.authorÀlvarez, G.
dc.contributor.authorIsabal, S.
dc.contributor.authorTeughels, W.
dc.contributor.authorLaleman, I.
dc.contributor.authorContreras Rebollo, María José
dc.contributor.authorIsbej Esposito, Lorena Pilar
dc.contributor.authorHuapaya, E.
dc.contributor.authorMendoza, G.
dc.contributor.authorMor, C.
dc.contributor.authorNart, J.
dc.contributor.authorBlanc, V.
dc.contributor.authorLeón, R.
dc.date.accessioned2026-01-19T18:35:55Z
dc.date.available2026-01-19T18:35:55Z
dc.date.issued2026
dc.description.abstractBackground The oral cavity is a known reservoir of antibiotic resistance genes (ARGs), but little is known about their subgingival distribution across health states and regions. Objective This study aimed to characterize and compare the subgingival resistome and mobile genetic elements (MGEs) in healthy subjects (HS) and periodontitis patients (PP) from Belgium, Chile, Peru and Spain. Design Subgingival samples pooled from the deepest site of each quadrant of 40 HS and 40 PP were analyzed via shotgun metagenomic sequencing. After human DNA depletion, the microbial composition was assessed with MetaPhlAn 4.0; ARGs were identified using MEGAHIT and AMRFinderPlus; and MGEs with MGEfinder. Results ARG richness was significantly higher in PP (mean 3.98) than in HS (2.15). PP from Peru showed more ARGs than HS from Chile and Spain. In total, 28 ARGs were found, conferring resistance to eight antibiotic classes. β-lactam, tetracycline and aminoglycoside resistance were more abundant in PP. Macrolide resistance was lower in Chilean samples than in Peruvian and Spanish ones. Additionally, 99 MGE-associated genes were detected, with 16 differing by diagnosis and 78 by country. Conclusions Subgingival resistome profiles vary significantly by periodontal status and geography, underscoring the influence of clinical and regional factors on antimicrobial resistance in the oral microbiome.
dc.fechaingreso.objetodigital2026-01-19
dc.format.extent13 páginas
dc.fuente.origenORCID
dc.identifier.doi10.1080/20002297.2025.2610588
dc.identifier.urihttps://doi.org/10.1080/20002297.2025.2610588
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/107758
dc.information.autorucEscuela de Medicina; Contreras Rebollo, María José; S/I; 1012433
dc.information.autorucEscuela de Medicina; Isbej Esposito, Lorena Pilar; 0000-0002-4272-8484; 1009697
dc.issue.numero1
dc.language.isoen
dc.nota.accesocontenido completo
dc.revistaJournal of Oral Microbiology
dc.rightsacceso abierto
dc.subjectAntibiotic resistance microbial
dc.subjectDental Plaque
dc.subjectMetagenomics
dc.subjectMicrobiome
dc.subjectMulticenter studies
dc.subjectPeriodontitis
dc.subject.ddc610
dc.titleCross-sectional comparative shotgun metagenomic analysis of the subgingival resistome in healthy subjects and patients with periodontitis from four countries
dc.typeartículo
dc.volumen18
sipa.codpersvinculados1012433
sipa.codpersvinculados1009697
sipa.trazabilidadORCID;2026-01-12
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