Compensatory Transcriptional Response of Fischerella thermalis to Thermal Damage of the Photosynthetic Electron Transfer Chain

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dc.catalogadorjca
dc.contributor.authorVergara-Barros, Pablo
dc.contributor.authorAlcorta Loyola, Jaime Andrés
dc.contributor.authorCasanova-Katny, Angélica
dc.contributor.authorNürnberg, Dennis J.
dc.contributor.authorDíez, Beatriz
dc.date.accessioned2023-06-27T13:19:34Z
dc.date.available2023-06-27T13:19:34Z
dc.date.issued2022
dc.description.abstractKey organisms in the environment, such as oxygenic photosynthetic primary producers (photosynthetic eukaryotes and cyanobacteria), are responsible for fixing most of the carbon globally. However, they are affected by environmental conditions, such as temperature, which in turn affect their distribution. Globally, the cyanobacterium Fischerella thermalis is one of the main primary producers in terrestrial hot springs with thermal gradients up to 60 °C, but the mechanisms by which F. thermalis maintains its photosynthetic activity at these high temperatures are not known. In this study, we used molecular approaches and bioinformatics, in addition to photophysiological analyses, to determine the genetic activity associated with the energy metabolism of F. thermalis both in situ and in high-temperature (40 °C to 65 °C) cultures. Our results show that photosynthesis of F. thermalis decays with temperature, while increased transcriptional activity of genes encoding photosystem II reaction center proteins, such as PsbA (D1), could help overcome thermal damage at up to 60 °C. We observed that F. thermalis tends to lose copies of the standard G4 D1 isoform while maintaining the recently described D1INT isoform, suggesting a preference for photoresistant isoforms in response to the thermal gradient. The transcriptional activity and metabolic characteristics of F. thermalis, as measured by metatranscriptomics, further suggest that carbon metabolism occurs in parallel with photosynthesis, thereby assisting in energy acquisition under high temperatures at which other photosynthetic organisms cannot survive. This study reveals that, to cope with the harsh conditions of hot springs, F. thermalis has several compensatory adaptations, and provides emerging evidence for mixotrophic metabolism as being potentially relevant to the thermotolerance of this species. Ultimately, this work increases our knowledge about thermal adaptation strategies of cyanobacteria.
dc.fechaingreso.objetodigital2023-06-27
dc.fuente.origenORCID
dc.identifier.doi10.3390/molecules27238515
dc.identifier.issn1420-3049
dc.identifier.urihttp://doi.org/10.3390/molecules27238515
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/73569
dc.information.autorucFacultad de Ciencias Biológicas;S/I;Alcorta Loyola, Jaime Andrés;187040
dc.issue.numero23
dc.language.isoen
dc.nota.accesoContenido completo
dc.revistaMolecules
dc.rightsacceso abierto
dc.subjectThermophiles
dc.subjectCyanobacteria
dc.subjectFischerella thermalis
dc.subjectPhotosynthesis
dc.subjectHot springs
dc.subjectPhotosystem II
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.titleCompensatory Transcriptional Response of Fischerella thermalis to Thermal Damage of the Photosynthetic Electron Transfer Chain
dc.typeartículo
sipa.codpersvinculados187040
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