Development of an implantable three-dimensional model of a functional pathogenic multispecies biofilm to study infected wounds

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dc.catalogadorgjm
dc.contributor.authorCárdenas Calderón, Camila Valentina
dc.contributor.authorVeloso Giménez, Valentina del Carmen
dc.contributor.authorGonzález, Tamara
dc.contributor.authorWozniak Banchero, Aniela
dc.contributor.authorGarcía, Patricia
dc.contributor.authorSan Martín, Sebastián
dc.contributor.authorVaras, Juan F.
dc.contributor.authorCarrasco-Wong, Ivo
dc.contributor.authorVera, Mario
dc.contributor.authorEgaña, José T.
dc.date.accessioned2024-01-30T15:23:31Z
dc.date.available2024-01-30T15:23:31Z
dc.date.issued2022
dc.description.abstractChronic wounds cannot heal due to impairment of regeneration, mainly caused by the persistent infection of multispecies biofilms. Still, the effects of biofilm wound infection and its interaction with the host are not fully described. We aimed to study functional biofilms in physiological conditions in vitro, and their potential effects in health and regeneration in vivo. Therefore, Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus faecalis were seeded in collagen-based scaffolds for dermal regeneration. After 24 h, scaffolds had bacterial loads depending on the initial inoculum, containing viable biofilms with antibiotic tolerance. Afterwards, scaffolds were implanted onto full skin wounds in mice, together with daily supervision and antibiotic treatment. Although all mice survived their health was affected, displaying fever and weight loss. After ten days, histomorphology of scaffolds showed high heterogeneity in samples and within groups. Wounds were strongly, mildly, or not infected according to colony forming units, and P. aeruginosa had higher identification frequency. Biofilm infection induced leucocyte infiltration and elevated interferon-γ and interleukin-10 in scaffolds, increase of size and weight of spleen and high systemic pro-calcitonin concentrations. This functional and implantable 3D biofilm model allows to study host response during infection, providing a useful tool for infected wounds therapy development.
dc.fechaingreso.objetodigital2024-06-04
dc.fuente.origenORCID
dc.identifier.doi10.1038/s41598-022-25569-5
dc.identifier.urihttp://www.scopus.com/inward/record.url?eid=2-s2.0-85144224586&partnerID=MN8TOARS
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/81071
dc.information.autorucEscuela de Medicina; Wozniak Banchero, Aniela; 0000-0001-9559-7660; 1008612
dc.language.isoen
dc.nota.accesoContenido completo
dc.rightsacceso abierto
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleDevelopment of an implantable three-dimensional model of a functional pathogenic multispecies biofilm to study infected wounds
dc.typeartículo
sipa.codpersvinculados1008612
sipa.trazabilidadORCID;2024-01-15
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