Development and analytical validation of real-time PCR for the detection of Streptococcus agalactiae in pregnant women.

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dc.contributor.authorEscobar, Daniel F.
dc.contributor.authorDiaz-Dinamarca, Diego A.
dc.contributor.authorBastías, Diego N.
dc.contributor.authorKalergis Parra, Alexis Mikes
dc.contributor.authorHernández, Carlos F.
dc.contributor.authorSoto, Daniel A.
dc.contributor.authorManzo, Ricardo A.
dc.contributor.authorAlarcón, Pedro I.
dc.contributor.authorPinto, Camila H.
dc.contributor.authorOberg-Bravo, Carolayn N.
dc.date.accessioned2020-06-16T16:10:38Z
dc.date.available2020-06-16T16:10:38Z
dc.date.issued2020
dc.date.updated2020-06-14T00:03:23Z
dc.description.abstractAbstract Background Group B Streptococcus (GBS) is the leading cause of invasive neonatal infection. In this study, we aimed to evaluate the analytical validation of qualitative real-time polymerase chain reaction (qPCR) as a means to detect GBS. Methods Genomic DNA (gDNA) was purified from 12 ATCC bacterial strains, two belonging to GBS and the remainder acting as negative controls. Additionally, gDNA was isolated from 21 strains of GBS from various serotypes (Ia, Ib and II-VIII). All gDNA was used to evaluate the analytical validation of the qPCR method employing a specific Taqman probe. Inclusivity, exclusivity, anticipated reportable range, the limit of detection and robustness were evaluated. The methods used are described in international guidelines and other existing reports. The performance of this qPCR method for detecting GBS was compared to other microbiological methods used with vaginal-rectal samples from pregnant women. Results Our qPCR method for detecting GBS was analytically validated. It has a limit of detection of 0.7 GE/μL and 100% analytical specificity. It detects all strains of GBS with the same level of performance as microbiological methods. Conclusion Data suggest that this qPCR method performs adequately as a means to detect GBS in vaginal-rectal swabs from pregnant women.
dc.identifier.citationBMC Pregnancy and Childbirth. 2020 Jun 09;20(1):352
dc.identifier.doi10.1186/s12884-020-03038-z
dc.identifier.urihttps://doi.org/10.1186/s12884-020-03038-z
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/29397
dc.issue.numeroNo. 352
dc.language.isoen
dc.nota.accesoContenido completo
dc.pagina.final10
dc.pagina.inicio1
dc.revistaBMC Pregnancy and Childbirthes_ES
dc.rightsacceso abierto
dc.rights.holderThe Author(s)
dc.subjectGroup B Streptococcuses_ES
dc.subjectqPCRes_ES
dc.subjectSurface immunogenic proteines_ES
dc.subjectAnalytical validationes_ES
dc.subjectBacterial detectiones_ES
dc.subject.ddc579.355
dc.subject.deweyBiologíaes_ES
dc.titleDevelopment and analytical validation of real-time PCR for the detection of Streptococcus agalactiae in pregnant women.es_ES
dc.typeartículo
dc.volumenVol. 20
sipa.codpersvinculados90610
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