The N-homologue LRR domain adopts a folding which explains the TMV-Cg-induced HR-like response in sensitive tobacco plants

Simple item page
dc.contributor.authorStange, Claudia
dc.contributor.authorMatus, Jose Tomas
dc.contributor.authorDominguez, Calixto
dc.contributor.authorPerez Acle, Tomas
dc.contributor.authorArce Johnson, Patricio
dc.date.accessioned2024-01-10T13:43:52Z
dc.date.available2024-01-10T13:43:52Z
dc.date.issued2008
dc.description.abstractFollowing leaf infection with the tobacco mosaic virus (TMV), Nicotiana species that carry the disease resistance N gene develop a hypersensitive response (HR) that blocks the systemic movement of the virus. TMV-sensitive tobacco plants that lack the N gene develop classical disease symptoms following infection with most of the tobamoviruses. However, upon infection with TMV-Cg, these p, ants display a HR-like response that is unable to limit viral spread. We previously identified the NH gene in sensitive plants; this gene is homologous to the resistance N gene and both belong to the TIR/NBS/LRR family. Isolation and analysis of the NH transcript enabled the prediction of the amino acid sequence in which we detected a leucine-rich repeat domain, proposed to be involved in pathogen recognition. This domain is found in four of five classes of pathogen resistant proteins, in which sequence and structural changes may generate different specificities. In order to study the possible functional role of the LRR domain in the HR-like response, we developed a comparative three-dimensional model for the NH and N gene products, by means of functional and structural domains recognition, secondary structure prediction, domain assignment through profile Hidden Markov Models (HMM) and molecular dynamics (MD) simulations. Based on our results we postulate that the NH protein could adopt a LRR fold with a functional role in the HR-like response. Our two reliable LRR three-dimensional models (N-LRR, NH-LRR) can be used as structural frameworks for future experiments in which the structure-function relationships regarding the protein-protein interaction process may be revealed. Evolutionary aspects of the N and NH genes in Nicotiana species are also discussed. (c) 2007 Elsevier Inc. All rights reserved.
dc.fechaingreso.objetodigital27-03-2024
dc.format.extent11 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.jmgm.2007.05.006
dc.identifier.eissn1873-4243
dc.identifier.issn1093-3263
dc.identifier.pubmedidMEDLINE:17631403
dc.identifier.urihttps://doi.org/10.1016/j.jmgm.2007.05.006
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/78779
dc.identifier.wosidWOS:000253185600006
dc.information.autorucCiencias Biológicas;Arce-Johnson P;S/I;54718
dc.information.autorucCiencias Biológicas;Domínguez C;S/I;1002862
dc.information.autorucCiencias Biológicas;Matus J;S/I;15841
dc.information.autorucCiencias Biológicas;Pérez-Acle T;S/I;90704
dc.information.autorucCiencias Biológicas;Stange C;S/I;6171
dc.issue.numero5
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final860
dc.pagina.inicio850
dc.publisherELSEVIER SCIENCE INC
dc.revistaJOURNAL OF MOLECULAR GRAPHICS & MODELLING
dc.rightsacceso restringido
dc.subjectNH gene
dc.subjectHR-like
dc.subjectTIR/NBS/LRR receptor
dc.subjectLRR
dc.subjectcomparative modelling
dc.subjecthypersensitive response
dc.subjectTMV
dc.subjectLEUCINE-RICH REPEATS
dc.subjectINHIBITING PROTEIN PGIP
dc.subjectDISEASE RESISTANCE
dc.subjectPOSITIVE SELECTION
dc.subjectSTRUCTURAL BASIS
dc.subjectGENE
dc.subjectVIRUS
dc.subjectIDENTIFICATION
dc.subjectSEQUENCE
dc.subjectSTRAIN
dc.subject.ods13 Climate Action
dc.subject.ods02 Zero Hunger
dc.subject.odspa13 Acción por el clima
dc.subject.odspa02 Hambre cero
dc.titleThe N-homologue LRR domain adopts a folding which explains the TMV-Cg-induced HR-like response in sensitive tobacco plants
dc.typeartículo
dc.volumen26
sipa.codpersvinculados54718
sipa.codpersvinculados1002862
sipa.codpersvinculados15841
sipa.codpersvinculados90704
sipa.codpersvinculados6171
sipa.indexWOS
sipa.trazabilidadCarga SIPA;09-01-2024
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
2024-03-27. The N-homologue LRR domain adopts a folding which explains the TMV-Cg-induced HR-like response in sensitive tobacco plants.pdf
Size:
3.34 KB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Artículos de revistas